Primary Phylogenies of Mauremys guangxiensis and Mauremys iversoni Inferred from DNA Sequences of Mitochondrial ND4 Gene and Nuclear c-mos Gene
Author of the article:HU Qiping1, HUANG Chengxin1, XU Shuru2, ZHANG Qiuming3*, MA Ning4, ZHONG Hanming5*
Author's Workplace：(1. Department of Cell Biology and Genetics, Guangxi Medical University, Nanning 530021, China; 2. Zhangzhou Health Vocational College, Zhangzhou, Fujian Province 363000, China; 3. Guangxi Fisheries Technology Extension Center, Nanning 530022, China; 4. Fisheries Research Institute of Guangxi, Nanning 530021, China; 5. Qinzhou Terrapin Association, Qinzhou, Guangxi Zhuang Autonomous Region 535000, China)
Key Words： ND4 gene; c-mos gene; Mauremys guangxiensis; Mauremys iversoni; phylogeny
Abstract：The phylogenies of Mauremys guangxiensis and Mauremys iversoni have been disputed for several years. To analyze the molecular phylogenies of M. guangxiensis, M. iversoni via detecting the partial mitochondrial ND4 gene and nuclear c-mos gene sequences of few M. guangxiensis, M. iversoni and M. mutica, and by combining the ND4 and c-mos gene sequences of other Mauremys species downloaded from GenBank, we inferred that from ND4 gene, the clades of M. guangxiensis were a branch of the clades of M. annamensis in NJ, MP and BI tree; the genetic distances was ranged 0.002~0.017 in each of these two species，and was ranged 0.000~0.035 between the two species. The later data was much less than that of the other species in the same genus (0.056~0.109), all above data indicated that M. guangxiensis was probably a synonymy with M. annamensis, or a subspecies of M. annamensis. Shown in NJ, MP and BI tree, M. iversoni located M. mutica proximally; the genetic distance between these two species was ranged 0.020~0.035, respectively, which were less than that of the other species in the same genus, while were much more than that of the other species in the same genus significantly; those data support that the relationship between M. iversoni and M. mutica is neither a species nor 2 different species; The locations of M. reevesii, M. nigricans, M. sinensis and M. annamensis shown in NJ, MP and BI tree suppoted that the genus of Chinemys, Ocadia and Annamemys should be merged into the genus Mauremys. Inferred from c-mos gene, there is no genetic distances among all Mauremys species, and no species can be distinguished in their NJ tree or MP tree; while according to the molecular phylogenies of genera or families, data of c-mos gene are credible evidences, and is consistent with the data of mitochondrial ND4 gene.