Identification Method of Nascent Sex Chromosomes
Author of the article:CHU Zhangqing1,2#, LUO Wei3#, XIA Yun1*
Author's Workplace：1.Chengdu Institute of Biology， Chinese Academy of Sciences， Chengdu 610041， China
2.University of Chinese Academy of Sciences，Beijing 100049， China
3.Ecological Security and Protection Key Laboratory of Sichuan Province， Mianyang Normal University，Mianyang， Sichuan Province 621000， China
Key Words：nascent sex chromosome;sex determination;sex?linked marker;sex chromosome transitions;vertebrate
Abstract：Sex chromosome evolution and sex determination mechanism has always been the intriguing aspect in vertebrate evolution research. In recent years， the concept of sexomics has been put forward. The sex determination mechanisms vary in vertebrates， especially， the species with nascent sex chromosome have more diverse evolutionary patterns. However， it is hard to distinguish the karyotype of nascent sex chromosome using traditional methods. This article illustrated the identification of sex‑linked molecular markers and the decision scheme of sex determination system through multiple dimensions， such as cytogenetic approach， DNA sequence in sex chromosome， the expression of RNA sequence， protein expression and so on. The future research will head toward the formation of sexomics by conducting a deeper research on sex determination system based on high‑throughput sequencing combined with genomics， proteomics and metabonomics. It is proposed that more model organisms and the evolutionary forces and molecular pathways underlying the evolution of sex determination will be found out.