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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2022 Vol.41 No.4

Microscopic Observation and Disease Symptoms of Neoneuromus ignobilis Larvae Infected by Beauveria bassiana
Author of the article:YUAN Jingze1, FENG Yi2, CAO Chengquan3, CHEN Hao3
Author's Workplace:1.College of Biological Resources and Environmental Sciences, Jishou University, Jishou, Hunan Province 416000, China
2.College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province 712100, China
3.College of Life Sciences, Leshan Normal University, Leshan, Sichuan Province 614004, China
Key Words:conidium;infection process;SEM;
Abstract:In this study, the infection processes of Beauveria bassiana conidia on the body surface and wound of the larvae of Neoneuromus ignobilis were observed by scanning electron microscopy (SEM) and ultra‑clear microscopy system. The results showed that after B. bassiana infection, the conidia were mainly distributed in the conjunctivae, back pleats, tubular projection of surface, depressions of surface and the setal alveolus of N. ignobilis. The conidia of B. bassiana germinated in the conjunctivae, back pleats, the setal alveolus and tubular projection of surface of N. ignobilis, and could invade the epidermis directly through the germ tube or after producing the appressorium. After inoculation, normal worms behaviour was slow after 48 h, and death began after 72-120 h. The mortality rate reached 83.33% after 96-120 h. By contrast, the symptom of B. bassiana wound infection model was basically the same as those infected on the body surface, while the number of deaths peaked about 12 h earlier than the latter. This study provides a theoretical basis for the prevention and control of B. bassiana infection during the artificial breeding of N. ignobilis.
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