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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2022 Vol.41 No.4

Genetic Diversity of Rana kukunoris Populations from Different Elevations Based on Four Mitochondrial Genes
Author of the article:ZHENG Haiqian, XU Zhiwang, XU Kangning, LI Hui, LI Shuran, ZHANG Yongpu*
Author's Workplace:College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang Province 325035, China
Key Words:mitochondrial genes;Qinghai?Tibet Plateau;amphibian;elevation;genetic diversity
Abstract:Rana kukunoris is an amphibian species endemically distributed in the humid environment of the Qinghai-Tibet Plateau at an elevation of 2 000-4 400 m. This study collected 100 individuals of R. kukunoris from 4 elevations (2 000 m, 2 600 m, 3 200 m and 3 800 m). The mitochondrial genes including COⅠcyt b12S rRNA and 16S rRNA of the individuals were obtained and combined to analyze the genetic diversity. The results showed that the number of polymorphic sites, haplotype diversity, and nucleotide diversity, and the average number of nucleotide differences of R. kukunoris population from 2 000 m and 2 600 m were higher than those from 3 200 m and 3 800 m. All the 4 populations showed high haplotype diversity and low nucleotide diversity. In addition, genetic differentiation mainly occurred between populations, and the results of neutral test and mismatch analysis indicated that R. kukunoris might not experience population expansion events. Positive selection sites were detected in the cyt b gene in the populations from 2 600 m, 3 200 m, and 3 800 m. In summary, the genetic diversity of R. kukunoris from 2 000 m and 2 600 m populations are higher than those from 3 200 m and 3 800 m populations, and there is a high degree of genetic differentiation among the 4 populations. Moreover, cyt b gene may play an important role in the adaptation of R. kukunoris to high‑elevation environments. These results contribute to understanding the high‑elevation adaptation of R. kukunoris.
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