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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2021 Vol.40 No.4

DNA Barcoding-Based Identification of Geometridae in the Laojunshan National Nature Reserve, Sichuan
Author of the article:WANG Hongmei1, ZHANG Xuelian1, WANG Lei1, YUE Bisong1, FENG Shenglin3, CHEN Benping3, MENG Yang1,2*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Nature History Museum of Sichuan University, Chengdu 610065, China;
3. Laojunshan National Nature Reserve Administration Bureau, Pingshan, Sichuan Province 645350, China
Key Words:Laojunshan National Nature Reserve; DNA barcoding; Geometridae; COⅠ gene
Abstract:To understand the resource of Geometridae (Lepidoptera) in the Laojunshan National Nature Reserve (Sichuan Province), a total of 2 402 specimens were collected from June to August, 2019 and from May to June, 2020. Molecular identification of specimens was carried out by using DNA barcoding technology. Based on the database of BOLD, Neighbor-Joining phylogenetic tree and Automatic Barcode Gap Discovery online tool were used to identify and delimit the 297 mitochondrial cytochrome c oxidase subunit Ⅰ sequences (COⅠ). Additionally, 367 COⅠsequences, including 70 sequences obtained from BOLD or GenBank databases, were used for genetic distance analysis. The results showed that the 297 sequences belonged to 191 species of Geometridae, and 95 of them were identified to the species level, and 35 were identified to the genus level. After excluding the sequences identified to the family level, the remaining sequences belonged to 92 genera. Among the 367 sequences, the intraspecific genetic distance was 0-4.36% with an average of 0.63%, and the interspecific genetic distance was 3.43%-19.12% with an average of 11.91%. This study suggested that the species of Geometridae can be well-differentiated by DNA barcoding technology based on COⅠ gene, and there were abundant species of Geometridae in the reserve.
2021,40(4): 404-414 收稿日期:2020-11-19
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