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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2021 Vol.40 No.2

Morphology and DNA Barcode of Nisia fuliginosa Yang & Hu, 1985, A New Pest of Schoenoplectus tabernaemontani
Author of the article:LYU Shasha1,2,3, CHEN Xiangsheng1,2,3*
Author's Workplace:1. The Institute of Entomology, Guizhou University, Guiyang 550025, China;
2. The Provincial Special Key Laboratory for Development and Utilization of Insect Resources, Guizhou University, Guiyang 550025, China;
3. The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guizhou University, Guiyang 550025, China
Key Words:Schoenoplectus tabernaemontani; Nisia fuliginosa; morphological characteristic; DNA barcode; damage symptoms
Abstract:Morphology and DNA barcode of Nisia fuliginosa, Yang & Hu, 1985, a new insect pest of Schoenoplectus tabernaemontani in the Caohai Wetland National Nature Reserve, Weining, Guizhou Province, were reported. The morphological characteristics of N. fuliginosa (eggs, nymphs and adults) were described in detail. The COⅠ sequence of N. fuliginosa were obtained by PCR, sequence compositions and genetic distances were analyzed, and the phylogenetic tree of 3 meenoplid species constructed by MEGA 6.0 using Neighbor-Joining methods was provided, Nisia sp. and Phaconeura sp. 2 species were chosen as outgroups. The results showed that the COⅠ sequences of 6 N. fuliginosa samples were clustered in the same evolutionary branches, and clearly distinguished from other species. The intraspecific genetic distances of N. fuliginosa was 0, whereas the interspecific genetic distances of the 3 meenoplid species was 0.166 8-0.236 1 and no overlapped genetic distance was detected between intraspecific and interspecific. Moreover, the damage symptoms of N. fuliginosa were introduced. This study suggests that the sequence of COⅠ gene can be used as a DNA barcode for the fast and accurate identification of the eggs, nymphs and adults of N. fuliginosa.
2021,40(2): 130-141 收稿日期:2020-07-04
丁永顺, 常志敏, 杨琳, 等. 2018. 乌桕新害虫——中华卡扁蜡蝉的形态及分子鉴定[J]. 林业科学研究, 31(6):69-75.
范铮, 孙培龙, 马新, 等. 2013. 水葱提取物的抗菌作用研究[J]. 食品科技, 38(2):214-217.
顾懿宁, 刘春林, 缪爱珠, 等. 2013. 水葱治疗良性前列腺增生的疗效[J]. 江苏医药, 39(8):974-975.
胡春林, 杨莲芳. 1993. 中国脉蜡蝉科初步名录附一新种(同翅目:蜡蝉总科)[J]. 昆虫分类学报, 15(1):35-40.
李凤山, 聂卉, 叶长虹. 1997. 黑颈鹤越冬期间植食性食物的显微分析[J]. 动物学研究, 18(1):51-57.
李志胜. 2002. 稻田生态系统杂草地生境节肢动物群落结构及其动态研究[D]. 福州:福建农林大学.
刘鹏岩, 傅承光. 1992. 河北水葱挥发油的GC-MS分析[J]. 分析测试通报, 11(3):4.
任珺, 付朝文, 陶玲, 等. 2011. 芦苇、菖蒲和水葱对水体中Zn~(2+)的富集效应研究[J]. 湿地科学, 9(4):322-327.
任珺, 陶玲, 杨倩, 等. 2010. 芦苇、菖蒲和水葱对水体中Cd富集能力的研究[J]. 农业环境科学学报, 29(9):1757-1762.
杨莲芳, 胡春林. 1985. 粒脉蜡蝉科四新种记述(同翅目:蜡蝉总科)[J]. 南京农业大学学报, 8(4):21-27.
杨倩. 2009. 三种湿地植物对污染水体中重金属离子的积累能力研究[D]. 兰州:兰州交通大学.
赵伟, 刘强. 2010. 中国特有植物四合木的昆虫群落与多样性特征[J]. 昆虫知识, 47(1):177-182.
赵伟, 刘强. 2011. 四合木(Tetraena mongolica)林地昆虫群落的组成、结构及多样性特征[J]. 生态学杂志, 30(11):168-175.
Bourgoin T. 2020. FLOW (Fulgoromorpha lists on the web):a world knowledge base dedicated to Fulgoromorpha. Version 8[DB/OL].[2020-12-30]. http://hemipteradatabasesorg/flow.
Liu H, Beckenbach AT. 1992. Evolution of the mitochondrial cytochrome oxidase Ⅱ gene among 10 orders of insects[J]. Molecular Phylogenetics and Evolution, 1(1):41-52.
Tamura K, Stecher G, Peterson D, et al. 2013. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 30(12):2725-2729.
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