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Your Position :Home->Past Journals Catalog->2021 Vol.40 No.2

Cloning and Expression Pattern of the Sensory Neuron Membrane Protein-Encoding Genes of Athetis dissimilis
Author of the article:SONG Zhiyu1, CHEN Shuangchen1, BAI Xiaojun2, DONG Junfeng1, SONG Yueqin1*
Author's Workplace:1. Forestry College, Henan University of Science and Technology, Luoyang, Henan Province 471000, China;
2. Yiyang Forestry Technical Guidance Station, Luoyang, Henan Province 471600, China
Key Words:Athetis dissimilis; sensory neuron membrane proteins; cloning; sequence analysis; expression profile
Abstract:Sensory neuron membrane proteins (SNMPs) in insects are critical peripheral olfactory proteins and act as markers for pheromone detection. In this study, two SNMP genes of Athetis dissimilis, named AdisSNMP1 and AdisSNMP2, were cloned and identified for the first time by quantitative real-time PCR. Sequence analysis showed that the encoding area of AdisSNMP1 gene is 1 572 bp in length, and the encoding area of AdisSNMP2 gene is 1 563 bp in length. Both genes encoding proteins are acidic, and the molecular weights are about 58 kD. Homology analysis showed that the consistency of SNMPgenes among the insects from the same order was high, the consistency of them with the species from different order was low, and the sequence consistency between SNMP1 and AdisSNMP2 was extremely low. The results of phylogenetic analysis also showed that the evolutionary relationship of SNMP genes of A. dissimilis with the insects from the same order were close. Quantitative real-time PCR showed that AdisSNMP1 was mainly expressed in the antennae of female and male individuals, whereas AdisSNMP2 was also expressed in other tissues. The study provides useful reference for further studies on the function of A. dissimilis SNMP genes.
2021,40(2): 121-129 收稿日期:2020-11-09
分类号:Q966
基金项目:国家自然科学基金项目(31701788);河南省科技攻关计划(农业领域)项目(202102110069;182102110182)
作者简介:宋智煜(1996-),研究生,主要研究领域为生物与分子生物学,E-mail:lylhsys@126.com
*通信作者:宋月芹,E-mail:songyueqin6@haust.edu.cn
参考文献:
郭婷婷, 门兴元, 于毅, 等. 2016a. 温度对双委夜蛾实验种群生长发育及繁殖的影响[J]. 昆虫学报, 59(8):865-870.
郭婷婷, 于志浩, 门兴元, 等. 2016b. 双委夜蛾不同虫态耐寒性及体内生化物质含量变化[J]. 昆虫学报, 59(12):1291-1297.
胡颖颖, 徐书法, 李微, 等. 2013. 中华蜜蜂感觉神经元膜蛋白基因克隆、组织表达分析及原核表达[J]. 昆虫学报, 56(1):9-17.
宋月芹, 李文亮, 刘顺通, 等. 2015. 双委夜蛾非典型嗅觉受体Orco的克隆、分子特征及表达[J]. 植物保护学报, 42(6):997-1003.
王振营, 石洁, 董金皋. 2012. 2011年黄淮海夏玉米区二点委夜蛾暴发危害的原因与防治对策[J]. 玉米科学, 20(1):132-134.
Benton R, Vannice KS, Gomez-Diaz C, et al. 2009. Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila[J]. Cell, 136(1):149-162.
Benton R, Vannice KS, Vosshall LB. 2007. An essential role for a CD36-related receptor in pheromone detection in Drosophila[J]. Nature, 450(7167):289-293.
Brito NF, Moreira MF, Melo ACA. 2016. A look inside odorant-binding proteins in insect chemoreception[J]. Journal of Insect Physiology, 95:51-65.
de Fouchier A, Walker WBIII, Montagné N, et al. 2017. Functional evolution of Lepidoptera olfactory receptors revealed by deorphanization of a moth repertoire[J/OL]. Nature Communications, 8:15709[2020-08-30]. https://doi.org/10.1038/ncomms15709.
Dong J, Song Y, Li W, et al. 2016. Identification of putative chemosensory receptor genes from the Athetis dissimilis antennal transcriptome[J/OL]. PLoS ONE, 11(1):e0147768[2020-08-30]. https://doi.org/10.1371/journal.pone.0147768.
Febbraio M, Silverstein RL. 2007. CD36:implications in cardiovascular disease[J]. The International Journal of Biochemistry & Cell Biology, 39(11):2012-2030.
Forstner M, Gohl T, Gondesen I, et al. 2008. Differential expression of SNMP-1 and SNMP-2 proteins in pheromone sensitive hairs of moths[J]. Chemical Senses, 33(3):291-299.
Gu SH, Yang RN, Guo MB, et al. 2013. Molecular identification and differential expression of sensory neuron memebrane proteins in the antennae of the black cutworm moth Agrotis ipsilon[J]. Journal of Insect Physiology, 59:430-443.
Herboso L, Talamillo A, Pérez C, et al. 2011. Expression of the scavenger receptor class B type Ⅰ (SR-BⅠ) family in Drosophila melanogaster[J]. International Journal of Developmental Biology, 55(6):603-611.
Hu YY, Xu SF, Wubie AJ, et al. 2013. Molecular characterization and tissue localization of sensory neuron membrane protein from Chinese honey bee, Apiscerana cerana (Hymenoptera:Apidae)[J]. Applied Entomology and Zoology, 48(4):533-545.
Huang XL, Liu L, Fang YQ, et al. 2016. Expression of a sensory neuron membrane protein SNMP2 in olfactory sensilla of codling moth Cydia pomonella (Lepidoptera:Tortricidae)[J]. Journal of Economic Entomology, 4(109):1907-1913.
Jin X, Ha TS, Smith DP. 2008. SNMP is a signaling component required for pheromone sensitivity in Drosophila[J]. Proceedings of the National Academy of Sciences of the United States of America, 105(31):10996-11001.
Kaupp UB. 2010. Olfactory signalling in vertebrates and insects:differences and commonalities[J]. Nature Reviews Neuroscience, 11(3):188-200.
Leal WS. 2013. Odorant reception in insects:roles of receptors, binding proteins, and degrading enzymes[J]. Annual Review of Entomology, 58:373-391.
Leal WS, Ishida Y, Pelletier J, et al. 2009. Olfactory proteins mediating chemical communication in the navel orangeworm moth, Amyelois transitella[J/OL]. PLoS ONE, 4(9):e7235[2020-08-31].https://doi.org/10.1371/journal.pone.0007235.
Levy E, Spahis S, Sinnett D, et al. 2007. Intestinal cholesterol transport proteins:an update and beyond[J]. Current Opinion in Lipidology, 18(3):310-318.
Li PY, Qin YC. 2011. Molecular cloning and characterization of sensory neuron membrane protein and expression pattern analysis in the diamondback moth, Plutella xylostella (Lepidoptera:Plutellidae)[J]. Applied Entomology and Zoology, 46(497):497-504.
Liu C, Zhang J, Liu Y, et al. 2014. Expression of SNMP1 and SNMP2 genes in antennal sensilla of Spodoptera exigua (Hübner)[J]. Archives of Insect Biochemistry and Physiology, 85(2):114-126.
Liu S, Qiao F, Liang QM, et al. 2013a. Molecular characterization of two sensory neuron membrane proteins from Chilo suppressalis (Lepidoptera:Pyralidae)[J]. Annals of the Entomological Society of America, 106(3):378-384.
Liu S, Zhang YR, Zhou WW, et al. 2013b. Identification and characterization of two sensory neuron membrane proteins from Cnaphalocrocis medinalis (Lepidoptera:Pyralidae)[J]. Archives of Insect Biochemistry and Physiology, 82(1):29-42.
Martin C, Chevrot M, Poirier H, et al. 2011. CD36 as a lipid sensor[J]. Physiology & Behavior, 105:36-42.
Montell C. 2009. A taste of the Drosophila gustatory receptors[J]. Current Opinion in Neurobiology, 19(4):345-353.
Nichols Z, Vogt RG. 2008.The SNMP/CD36 gene family in Diptera, Hymenoptera and Coleoptera:Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae, Aedes aegypti, Apis mellifera, and Tribolium castaneum[J]. Insect Biochemistry and Molecular Biology, 38(4):398-415.
Pregitzer P, Greschista M, Breer H, et al. 2014. The sensory neuron membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system[J]. Insect Molecular Biology, 23(6):733-742.
Rogers ME, Krieger J, Vogt RG. 2001a. Antennal SNMPs (sensory neuron membrane proteins) of Lepidoptera define a unique family of invertebrate CD36-like proteins[J]. Journal of Neurobiology, 49(1):47-61.
Rogers ME, Steinbrecht RA, Vogt RG. 2001b. Expression of SNMP-1 in olfactory neurons and sensilla of male and female antennae of the silkmoth Antheraea polyphemus[J]. Cell and Tissue Research, 303(3):433-446.
Rogers ME, Sun M, Lerner MR, et al. 1997. Snmp-1, a novel membrane protein of olfactory neurons of the silk moth Antheraea polyphemus with homology to the CD36 family of membrane proteins[J]. Journal of Biological Chemistry, 272(23):14792-14799.
Sun L, Wang Q, Zhang Y, et al. 2019. Expression patterns and colocalization of two sensory neurone membrane proteins in Ectropis oblique Prout, a geometrid moth pest that uses Type-Ⅱ sex pheromones[J]. Insect Molecular Biology, 28(3):342-354.
Sun L, Xiao HJ, Gu SH, et al. 2014. Perception of potential sex pheromones and host-associated volatiles in the cotton plant bug, Adelphocoris fasciaticollis (Hemiptera:Miridae):morphology and electrophysiology[J]. Applied Entomology and Zoology, 49(1):43-57.
Vogt RG, Miller NE, Litvack R, et al. 2009. The insect SNMP gene family[J]. Insect Biochemistry and Molecular Biology, 39(7):448-456.
Yang HY, Ning SY, Sun X, et al. 2020. Identification and characterization of two sensory neuron membrane proteins from onion maggot (Diptera:Anthomyiidae)[J]. Journal of Economic Entomology, 113(1):418-426.
Yang K, Huang LQ, Ning C, et al. 2017. Two single-point mutations shift the ligand selectivity of a pheromone receptor between two closely related moth species[J/OL]. eLife, 6:e29100[2020-07-30].https://doi.org/10.7554/eLife.29100.001.
Zhang HJ, Xu W, Sun LN, et al. 2018. Functional characterization of sensory neuron membrane proteins (SNMPs)[J/OL]. BioRxiv:262154[2020-07-30].https://doi.org/10.1001/262154.
Zhang J, Walker WB, Wang G. 2015. Pheromone reception in moths:from molecules to behaviors[J]. Progress in Molecular Biology and Translational Science, 130:109-128.
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