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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2021 Vol.40 No.1

A Mouse Model of Light-Controlled Cardiac Pacing
Author of the article:GE Yan, WANG Wei, LIU Jieyu, ZENG Bo*
Author's Workplace:Key Laboratory of Medical Electrophysiology, Ministry of Education, Insitute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan Province 646000, China
Key Words:optogenetics; light-controlled cardiac pacing; channelrhodopsin-2; arrhythmia
Abstract:Electrical stimulation is a standard technique for controlling cardiac beating frequency. However, in experimental studies, this method has critical technical limitations, such as local electrolysis effect, limited stimulation frequency and duration, stimulating all cells within the target range, and lack of selectivity on cardiomyocytes. Based on optogenetic technology, transgenic mice with specific expression of photoactivated cationic channel channelrhodopsin-2 (ChR2) in cardiomyocytes were constructed, and then blue light at specific frequency was used to stimulate cardiomyocytes and depolarize the membrane potential which can trigger the action potential and control the cardiac pacing. When the frequency of blue-light stimulation was lower than the spontaneous heart-beating frequency of mice, the blue-light stimulation caused temporary cardiac arrhythmia, while the heart beating of mice returned to normal after the removal of blue-light illumination. When the frequency of blue-light stimulation was higher than the spontaneous heart rate of the mice, the heart beats of mice were completely controlled by blue-light stimulation, and returned to the spontaneous beating state after removing the blue light. Moreover, mouse hearts failed to beat autonomously could also be paced by blue light, and ChR2-deficient mice showed no response to blue-light stimulation. Therefore, optogenetics-based light-controlled cardiac-pacing technique is experimentally demonstrated in this study, which has very important values' in cardiac electrophysiology, especially in the study of arrhythmias.
2021,40(1): 39-45 收稿日期:2020-08-22
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