Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2020 Vol.39 No.6

Relationship Between Maximal Phytohemagglutinin-P Response Value and Hematological Indices, and Effects of Temperatures on the Response Patterns of Trachemys scripta elegans
Author of the article:WU Wenxin, WU Haiyan, YANG Yuqing, FENG Wenhong, XU Yangyi, DIAO Yingzhu, WANG Yin, ZHANG Zhiqiang*
Author's Workplace:College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Key Words:Trachemys scripta elegan; temperature; phytohemagglutinin-P; lymphocytes; immunological ecology
Abstract:To explore the relationship between maximal phytohemagglutinin-P (PHA-P) response value and hematological indices in the red-eared sliders (Trachemys scripta elegans), and the effects of temperatures on their PHA-P response patterns, variations of different subtypes of leukocyte and plasma bactericidal capacity from blood prior to injection and the maximal PHA-P response value were measured with optimal PHA-P injection concentration at 22 ℃, and the PHA-P response patterns to optimal PHA-P injection concentration were compared after 6 weeks acclimation of 20 ℃, 24 ℃, 28 ℃, and 32 ℃. Under the conditions of 22 ℃ water temperature, T. scripta elegans was treated with 1.0 g·L-1, 2.5 g·L-1, and 5.0 g·L-1 concentration of PHA-P solutions, the responsiveness to PHA-P peaked at 24 h, 48 h, and 24 h after injection, respectively, and the concentration of 2.5 g·L-1 was selected as the optimal, and the percentage of lymphocytes was significantly increased (P<0.05), but no significant differences were found for other subtypes of leukocyte, ratio of neutrophils to lymphocytes, and plasma bactericidal capacity from circulation (P>0.05) while reaching the peak. After the turtles were treated with different water temperatures of 20 ℃, 24 ℃, 28 ℃, and 32 ℃ for 6 weeks, they were injected with 2.5 g·L-1 PHA-P concentration, respectively. The responsiveness to PHA-P peaked at 48 h, 24 h, 24 h, and 24 h for 20 ℃, 24 ℃, 28 ℃ and 32 ℃ group, respectively, and no significant differences were found for the maximal PHA-P values among different temperature groups (P>0.05). From 24 h to 48 h postinjection, the responsiveness to PHA-P was increased at 20 ℃ group, decreased at 32 ℃ group, and remained relatively stable at 24 ℃ and 28 ℃ group, but no significant differences were found. In T. scripta elegans, the responsiveness to PHA-P solution is related to the increased number of lymphocytes, and shifts forward with arising temperatures within moderate temperature ranges for their survivals, these variations may in advance activate adaptive immunity to enhance their adaptability to higher temperature.
2020,39(6): 639-645 收稿日期:2020-05-29
高慧清, 张琼文, 孙思琪, 等. 2017. 变态期花背蟾蜍蝌蚪对植物血凝素的反应模式及其生态学意义[J]. 生态学杂志, 36(9): 2542-2548.
刘哲. 2015. 两种龟呼吸代谢和特殊热动力作用的比较[D]. 南京: 南京师范大学.
彭兵龙, 张昊滨, 陈可为, 等. 2019. 中华蟾蜍足垫部位对植物血凝素的反应及其组织学和血液学变化[J]. 动物学杂志, 54(1): 87-98.
吴琼, 戚琴芹, 耿军, 等. 2017. 不同孵化温度对中华鳖新生幼体免疫能力的影响[J]. 杭州师范大学学报(自然科学版), 16(5): 514-517.
张志强, 黄淑丽, 赵志军. 2015. 不同繁殖状态雌性黑线仓鼠对植物血凝素的反应模式[J]. 兽类学报, 35(1): 74-79.
张志强, 王泽洋, 许洋溢, 等. 2020. 黑斑侧褶蛙变态前后身体大小的变化及对植物血凝素的反应模式[J]. 安徽农业大学学报(自然科学版), 47(4): 519-523.
张志强. 2015. 动物生态学研究中免疫学参数的选择及其优缺点分析[J]. 四川动物, 34(1): 145-148.
周婷. 1997. 红耳龟的生物学及人工养殖[J]. 四川动物, 16(2): 96.
周赞, 蔡梦思, 刘虞, 等. 2016. 红耳滑龟和中华草龟幼体体温与运动的热依赖性、补偿生长及免疫力[J]. 生态学报, 36(21): 7014-7022.
Brock PM, Murdock CC, Martin LB. 2014. The history of ecoimmunology and its integration with disease ecology[J]. Integrative and Comparative Biology, 54(3): 353-362.
Davis AK, Maney DL, Maerz JC. 2008. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists[J]. Functional Ecology, 22(5): 760-772.
Finger JW, Amanda AL, Thomson PC, et al. 2013. Using phytohaemagglutinin to determine immune responsiveness in saltwater crocodiles (Crocodylus porosus)[J]. Australia Journal of Zoology, 61(4): 301-311.
Goto N, Kodama H, Okada K, et al. 1978. Suppression of phytohemagglutinin skin response in thymectomized chickens[J]. Poultry Science, 57(1): 246-250.
Martin LB, Han P, Lewittes J, et al. 2006. Phytohemagglutinin-induced skin swelling in birds: histological support for a classic immunoecological technique[J]. Functional Ecology, 20(2): 290-299.
Merlo JL, Cutrera AP, Zenuto RR. 2014. Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents[J]. Canadian Journal of Zoology, 92(8): 689-697.
Palackdharry S, Sadd BM, Vogel LA, et al. 2017. The effect of environmental temperature on reptilian peripheral blood B cell functions[J]. Hormones and Behavior, 88: 87-94.
Smits J, Bortolotti G, Tella J. 1999. Simplifying the phytohemagglutinin skin-testing technique in studies of avian immunocompetence[J]. Functional Ecology, 13(4): 567-572.
Zamora-Camacho FJ. 2019. Integrating time progression in ecoimmunology studies: beyond immune response intensity[J]. Current Zoology, 65(2): 205-212.
Zhang ZQ, Jin CC, Qu KS, et al. 2017. Immune responsiveness to phytohemagglutinin displays species but not sex differences in three anuran species[J/OL]. PeerJ, 5: e3181[2020-02-10].
Zhang ZQ, Zhao ZJ. 2015. Correlations between phytohemagglutinin response and leukocyte profile, and bactericidal capacity in a wild rodent[J]. Integrative Zoology, 10(3): 302-310.
Zimmerman LM, Carter AW, Bowden RM, et al. 2017. Immunocompetence in a long-lived ectothermic vertebrate is temperature dependent but shows no decline in older adults[J]. Functional Ecology, 31(7): 1383-1389.
Zimmerman LM, Paitz RT, Vogel LA, et al. 2010. Variation in the seasonal patterns of innate and adaptive immunity in the red-eared slider (Trachemys scripta)[J]. Journal of Experimental Biology, 213(9): 1477-1483.
CopyRight©2021 Editorial Office of Sichuan Journal of Zoology 蜀ICP备08107403号-3