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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2020 Vol.39 No.6

Relationship Between Maximal Phytohemagglutinin-P Response Value and Hematological Indices, and Effects of Temperatures on the Response Patterns of Trachemys scripta elegans
Author of the article:WU Wenxin, WU Haiyan, YANG Yuqing, FENG Wenhong, XU Yangyi, DIAO Yingzhu, WANG Yin, ZHANG Zhiqiang*
Author's Workplace:College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Key Words:Trachemys scripta elegan; temperature; phytohemagglutinin-P; lymphocytes; immunological ecology
Abstract:To explore the relationship between maximal phytohemagglutinin-P (PHA-P) response value and hematological indices in the red-eared sliders (Trachemys scripta elegans), and the effects of temperatures on their PHA-P response patterns, variations of different subtypes of leukocyte and plasma bactericidal capacity from blood prior to injection and the maximal PHA-P response value were measured with optimal PHA-P injection concentration at 22 ℃, and the PHA-P response patterns to optimal PHA-P injection concentration were compared after 6 weeks acclimation of 20 ℃, 24 ℃, 28 ℃, and 32 ℃. Under the conditions of 22 ℃ water temperature, T. scripta elegans was treated with 1.0 g·L-1, 2.5 g·L-1, and 5.0 g·L-1 concentration of PHA-P solutions, the responsiveness to PHA-P peaked at 24 h, 48 h, and 24 h after injection, respectively, and the concentration of 2.5 g·L-1 was selected as the optimal, and the percentage of lymphocytes was significantly increased (P<0.05), but no significant differences were found for other subtypes of leukocyte, ratio of neutrophils to lymphocytes, and plasma bactericidal capacity from circulation (P>0.05) while reaching the peak. After the turtles were treated with different water temperatures of 20 ℃, 24 ℃, 28 ℃, and 32 ℃ for 6 weeks, they were injected with 2.5 g·L-1 PHA-P concentration, respectively. The responsiveness to PHA-P peaked at 48 h, 24 h, 24 h, and 24 h for 20 ℃, 24 ℃, 28 ℃ and 32 ℃ group, respectively, and no significant differences were found for the maximal PHA-P values among different temperature groups (P>0.05). From 24 h to 48 h postinjection, the responsiveness to PHA-P was increased at 20 ℃ group, decreased at 32 ℃ group, and remained relatively stable at 24 ℃ and 28 ℃ group, but no significant differences were found. In T. scripta elegans, the responsiveness to PHA-P solution is related to the increased number of lymphocytes, and shifts forward with arising temperatures within moderate temperature ranges for their survivals, these variations may in advance activate adaptive immunity to enhance their adaptability to higher temperature.
2020,39(6): 639-645 收稿日期:2020-05-29
分类号:Q959.6;Q958.1;Q939.91
基金项目:国家自然科学基金项目(31170379);安徽省舒城县"三区"科技人才项目(2020);安徽农业大学2019年省级大学生创新创业训练计划项目创新训练项目(201910364042);安徽农业大学2020年省级质量工程项目教学改革研究重点项目(062)
作者简介:吴文欣(1999-),女,本科生,研究方向为水产养殖学,E-mail:1335747347@qq.com
*通信作者:张志强,教授,E-mail:zzq-003@163.com
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