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          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2020 Vol.39 No.3

Molecular Cloning and Expression Pattern of the DjPLCβ4 Gene in Dugesia japonica During Embryonic Development
Author of the article:ZHEN Hui, ZHAO Bosheng*
Author's Workplace:Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, Shandong Province 255000, China
Key Words:Dugesia japonica; DjPLCβ4 gene; whole-mount in situ hybridization; embryonic development
Abstract:The β isoform of phospholipase C (PLCβ) is crucial for the modulation of synaptic transmission and plasticity. As a bilaterally symmetrical animal with the earliest emergence of three germ layers, the planarian (Dugesia japonica) is an important model organism for studying the mechanisms of development and evolution. To explore the expression the DjPLCβ4 gene during the embryonic development, the DjPLCβ4 cDNA of D. japonica was obtained by RACE and RT-PCR. The expression pattern of DjPLCβ4 gene was detected by whole-mount in situ hybridization. The results demonstrated that the DjPLCβ4 gene was 3 245 bp in length and encoding 969 amino acids. The result of phylogenetic analysis showed that the planarian DjPLCβ4 might be the archetype of the bilateral species. DjPLCβ4 gene expressed in the embryonic pharynx and germ band at the stage 3 of embryonic development. Along with the embryonic development, the number of DjPLCβ4-positive embryonic cells was increased and mainly distributed in the neurons blastema, the nervous system regions and the brain of the fully hatched larvae and intact animals. These results suggested that the DjPLCβ4 gene participates in the formation and differentiation of the neural primordium during the embryonic development of planarian.
2020,39(3): 249-257 收稿日期:2019-12-03
分类号:Q959.151;Q78
基金项目:国家自然科学基金项目(31970430)
作者简介:甄辉,博士研究生,研究方向:发育生物学,E-mail:zhenhui1013@126.com
*通信作者:赵博生,教授,博导,E-mail:zhaobosheng@sdut.edu.cn
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