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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2020 Vol.39 No.1

Cloning of Axis porcinus MHC-DOA Gene and the Genetic Diversity of Its Exon 2 Region
Author of the article:SHAO Huanhuan1,2#, DENG Jiabo3#, WU Linfeng1, NIU Lili3, YU Jianqiu3, TAN Xuemei1*
Author's Workplace:1. Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. College of Life Sciences, Sichuan Normal University, Chengdu 610101, China;
3. Chengdu Zoo, Chengdu 610081, China
Key Words:Axis porcinus; MHC-DOA; exon 2; gene polymorphism
Abstract:The genes encoding the major histocompatibility complex (MHC) are important in the immune system of vertebrates. MHC-DOA gene is a non-classical gene of MHC class Ⅱ, of which exon 2 has rich polymorphisms. Hog deer (Axis porcinus) is an extremely endangered species in China. In this study, total RNA was extracted from the blood of A. porcinus and then the MHC-DOA gene was cloned by PCR from cDNA template. Meanwhile, the exon 2 of MHC-DOA genes in 38 A. porcinus from Chengdu Zoo were obtained by PCR and DNA-sequencing, followed by genetic polymorphism analysis. The results showed that the MHC-DOA gene of A. porcinus contained a 753 bp open reading frame, encoding a protein with a length of 250 amino acid residues. Sequence analysis predicted that DOA protein had high amino acids similarity (98.4%) with Cervus elaphus hippelaphus. A total of 10 haplotypes of DOA exon 2 were detected from the 38 individuals, indicating a medium level of genetic diversity in A. porcinus. Nucleotides insert and deletion were observed in MHC-DOA exon 2 gene, and these resulted in the change of MHC-DOA amino acid sequence. This study has significant importance in understanding the population genetic structure and the protection of A. porcinus in the future.
2020,39(1): 81-87 收稿日期:2019-03-14
分类号:Q959.842;Q987.1
基金项目:成都大熊猫繁育研究基金项目(CPF-2010-02)
作者简介:邵欢欢(1984-),男,博士,讲师,从事分子遗传学研究,E-mail:wsshforget@163.com;邓家波(1974-),男,硕士,高级兽医师,从事野生动物的研究,E-mail:565211989@qq.com
*通信作者:谭雪梅,讲师,主要从事动物分子遗传学研究,E-mail:txmyyf@scu.edu.cn
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