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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.6

Isolation and Identification of Bacillus with Antimicrobial Activity from the Lakes of Yunnan, Guizhou, and Sichuan Provinces
Author of the article:ZHANG Junli, LIU Chaolan, GUO Yidong, LIN Jiafu*
Author's Workplace:Sichuan Industrial Institute of Antibiotics, Chengdu University, Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, Chengdu 610052, China
Key Words:lake sediments; Bacillus; animal pathogens; antibacterial activity
Abstract:To test the antimicrobial activities of Bacillus species, a total of 244 bacterial strains were previously isolated from the sediments of lakes in Yunnan, Guizhou, and Sichuan provinces of China. The strains were identified by sequencing the 16S rRNA gene. After fermentation, the antimicrobial activities of the secondary metabolites of Bacillus was determined by using 4 strains of common animal pathogenic bacteria, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. The fermented products of strains were analyzed by High Performance Liquid Chromatography-Mass Spectrometry. The results showed that 47 isolates from 9 lake samples were identified as Bacillus, and 13 of which were found to have antimicrobial activities by producing the lipopeptide antibiotics. This study expands the current understanding of Bacillus species resources, lays a theoretical foundation for further studies on the secondary metabolites of Bacillus, and also opens a new avenue for the discovery of antibiotics against animal pathogens.
2019,38(6): 686-694 收稿日期:2019-01-27
分类号:R978.1
基金项目:中国湖泊微生物多样性及资源调查项目(2017FY100301);四川省医药微生物共享服务平台建设项目(2019JDPT0012);四川省教育厅科研项目(18ZB0137);四川省科技计划项目(2019YJ0282);四川省环境保护科技计划项目(2018HB27)
作者简介:张君利(1993-),硕士研究生,主要从事微生物药物研究,E-mail:1159904223@qq.com
*通信作者:林家富,E-mail:linjiafu@cdu.edu.cn
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