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Issue:ISSN 1000-7083
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.6

Study of the Regulatory Role of Krüppel-like Factor 4 in Mesenchymal Stem Cells of Ailuropoda melanoleuca
Author of the article:WU Hongjuan1, LIU Yuliang2*, LIANG Yutong1, SHEN Fujun2, HOU Rong2, PENG Rui1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Chengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Academy of Giant Panda, Chengdu 610081, China
Key Words:Ailuropoda melanoleuca; mesenchymal stem cells; Krüppel-like factor 4
Abstract:Giant panda (Ailuropoda melanoleuca) is one of the most well-known endangered species in China. Study on the molecule and gene function of giant panda genes contributes to better understanding of this species. Krüppel-like factor 4 (KLF4) is an important transcriptional factor and plays various roles in many physiological processes including cell cycle, cell growth, cell migration, cell differentiation, cell apoptosis and maintenance of normal tissue homeostasis. In addition, it is also an essential regulator during the process of somatic cell reprogramming. To further explore the role of Klf4 gene in giant panda, the full-length cDNA of giant panda Klf4 gene was cloned from mesenchymal stem cells (MSCs) using the rapid amplification cDNA ends technique, and then the structure characteristics were analyzed. Subsequently, Klf4 gene was overexpressed in the giant panda MSCs followed by exploring its regulatory role using RNA-seq. The results showed that giant panda KLF4 was conserved during evolution, and played important physiological roles in different biological processes including cell proliferation, cell apoptosis, RNA transport and protein biosynthesis. These findings lay a foundation for further studying giant pandas at molecular level and contribute to the future protection of this species.
2019,38(6): 630-637 收稿日期:2019-03-25
分类号:Q813;Q959.8
基金项目:成都大熊猫繁育研究基金会项目"大熊猫诱导多潜能干细胞库的建立"(CPF2017-16)
作者简介:吴宏娟,女,硕士研究生,研究方向:细胞生物学,E-mail:310911949@qq.com
*通信作者:刘玉良,研究员,E-mail:liuyuliang@panda.org.cn;彭锐,E-mail:pengrui@scu.edu.cn
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