Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2019 Vol.38 No.6

Study of the Regulatory Role of Krüppel-like Factor 4 in Mesenchymal Stem Cells of Ailuropoda melanoleuca
Author of the article:WU Hongjuan1, LIU Yuliang2*, LIANG Yutong1, SHEN Fujun2, HOU Rong2, PENG Rui1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Chengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Academy of Giant Panda, Chengdu 610081, China
Key Words:Ailuropoda melanoleuca; mesenchymal stem cells; Krüppel-like factor 4
Abstract:Giant panda (Ailuropoda melanoleuca) is one of the most well-known endangered species in China. Study on the molecule and gene function of giant panda genes contributes to better understanding of this species. Krüppel-like factor 4 (KLF4) is an important transcriptional factor and plays various roles in many physiological processes including cell cycle, cell growth, cell migration, cell differentiation, cell apoptosis and maintenance of normal tissue homeostasis. In addition, it is also an essential regulator during the process of somatic cell reprogramming. To further explore the role of Klf4 gene in giant panda, the full-length cDNA of giant panda Klf4 gene was cloned from mesenchymal stem cells (MSCs) using the rapid amplification cDNA ends technique, and then the structure characteristics were analyzed. Subsequently, Klf4 gene was overexpressed in the giant panda MSCs followed by exploring its regulatory role using RNA-seq. The results showed that giant panda KLF4 was conserved during evolution, and played important physiological roles in different biological processes including cell proliferation, cell apoptosis, RNA transport and protein biosynthesis. These findings lay a foundation for further studying giant pandas at molecular level and contribute to the future protection of this species.
2019,38(6): 630-637 收稿日期:2019-03-25
李静. 2017. 大熊猫肠道放线菌的种群组成及多样性分析[J]. 微生物学通报, 44(5):1138-1148.
Ambrish R, Alper K, Yang Z. 2010. I-TASSER:a unified platform for automated protein structure and function prediction[J]. Nature Protocols, 5(4):725-738.
Chen X, Whitney EM, Gao SY,et al. 2011. Transcriptional profiling of Krüppel-like factor 4 reveals a function in cell cycle regulation and epithelial differentiation[J]. Journal of Molecular Biology, 326(3):665-677.
Ghaleb AM, Yang VW. 2017. Krüppel-like factor 4 (KLF4):what we currently know[J]. Gene, 611:27-37.
Gupta A, Kailasam S, Bansal M. 2016. Insights into the structural dynamics of nucleocytoplasmic transport of tRNA by exportin-t[J]. Biophysical Journal, 110(6):1264-1279.
Kang D, Li J. 2018. Role of nature reserves in giant panda protection[J]. Environmental Science & Pollution Research, 25(5):4474-4478.
Kang D, Wang X, Li J. 2017. Resting site use of giant pandas in Wanglang Nature Reserve[J]. Scientific Reports, 7(1):13809. DOI:10.1038/s41598-017-14315-x.
Kaori IH, Hiroyuki S, Mari N, et al. 2014. KLF4-dependent epigenetic remodeling modulates podocyte phenotypes and attenuates proteinuria[J]. Journal of Clinical Investigation, 124(6):2523-2537.
Khacho M, Mekhail K, Pilonlarose K, et al. 2008. eEF1A is a novel component of the mammalian nuclear protein export machinery[J]. Molecular Biology of the Cell, 19:5296-5308.
Kumar S, Stecher G, Tamura K. 2016. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology & Evolution, 33(7):1870-1874.
Li R, Fan W, Tian G, et al. 2010. The sequence and de novo assembly of the giant panda genome[J]. Nature, 463(7279):311-317.
Liu YL, Liu Y, Yie SM, et al. 2013. Characteristics of mesenchymal stem cells isolated from bone marrow of giant panda[J]. Stem Cells & Development, 22(17):2394-2401.
Mallipattu SK, Estrada CC, He JC. 2016. The critical role of Krüppel-like factors in kidney disease[J]. American Journal of Physiology Renal Physiology, 312(2):259-265.
Qiu J, Yu L, Zhang X, et al. 2015. Asiaticoside attenuates lipopolysaccharide-induced acute lung injury via down-regulation of NF-κB signaling pathway[J]. International Immunopharmacology, 26(1):181-187.
Schuh R, Aicher W, Gaul U, et al. 1986. A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene[J]. Cell, 47(6):1025-1032.
Shatat MA, Hongmei T, Rongli Z,et al. 2014. Endothelial Krüppel-like factor 4 modulates pulmonary arterial hypertension[J]. American Journal of Respiratory Cell & Molecular Biology, 50(3):647-653.
Shen G, Feng C, Xie Z,et al. 2010. Proposed conservation landscape for giant pandas in the Minshan Mountains, China[J]. Conservation Biology, 22(5):1144-1153.
Takashi A, Kojiro Y, Masato N, et al. 2008. Generation of pluripotent stem cells from adult mouse liver and stomach cells[J]. Science, 321(5889):699-702.
Wang SH, Qu XB, Zhao RCH. 2012. Clinical applications of mesenchymal stem cells[J]. Journal of Hematology & Oncology, 5(1):19. DOI:10.1186/1756-8722-5-19.
Wassmann S, Wassmann K, Jung A, et al. 2007. Induction of p53 by GKLF is essential for inhibition of proliferation of vascular smooth muscle cells[J]. Journal of Molecular & Cellular Cardiology, 43(3):301-307.
Wei FW, Hu YB, Li Y, et al. 2015. Giant pandas are not an evolutionary cul-de-sac:evidence from multidisciplinary research[J]. Molecular Biology & Evolution, 32(1):4-12.
Yang D, Zhang Z, Liang S,et al. 2017. A novel role of Krüppel-like factor 4 in Zhikong scallop Chlamys farreri during spermatogenesis[J]. PLoS ONE, 12(6):e0180351. DOI:10.1371/journal.pone.0180351.
Yang J, Yan R, Roy A, et al. 2015. The I-TASSER Suite:protein structure and function prediction[J]. Nature Methods, 12(1):7-8.
Zhang JS, Daszak P, Huang HL, et al. 2008. Parasite threat to panda conservation[J]. EcoHealth, 5(1):6-9.
Zhang Y, Wang Y, Liu Y,et al. 2013. Krüppel-like factor 4 transcriptionally regulates TGF-β1 and contributes to cardiac myofibroblast differentiation[J]. PLoS ONE, 8(4):e63424. DOI:10.1371/journal.pone.0063424.
Zhang Y. 2008. I-TASSER server for protein 3D structure prediction[J]. BMC Bioinformatics, 9:40. DOI:10.1186/1471-2105-9-40.
CopyRight©2020 Editorial Office of Sichuan Journal of Zoology