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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.5

Characterization of Microsatellite DNA Loci and Design of Candidate Primers to Amplify These Regions for Phrynocephalus forsythii by Using 454 GS FLX
Author of the article:SONG Qi1,2, GUO Xianguang1, CHEN Dali3*
Author's Workplace:1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610064, China
Key Words:Phrynocephalus forsythii; genome; microsatellite; distribution; primer sequence
Abstract:The Forsyth's toad-headed agama, Phrynocephalus forsythii, is an endemic small lizard in the Tarim Basin in northwest China. The genome data of P. forsythii was generated using Roche 454 sequencing platform, and 55 909 high quality sequences were obtained. A total of 12 109 simple sequence repeats (SSRs) with 1-6 bp nucleotide motifs were identified by using Krait. Among the different repeat types of screened microsatellite DNA, tetranucleotide, having 4 037 (33.34%), was the most common repeat unit followed by the di-(28.09%), tri-(18.72%), mono-(13.91%), penta-(4.48%) and hexanucleotides (1.46%). Additionally, C, AC, AAC, AAAT, AAAAT, and AACCCT were the most common repeat units among the mono-hexanucleotides, respectively. In the genome of P. forsythii, there were 10 predominant repeat types, including AC, AAAT, C, AG, A, AAC, AAT, AAAC, ACC and ACG. One hundred pairs of candidate primers containing a part of tri-and tetranucleotide repeat types were designed to amplify the microsatellite DNA loci in P. forsythii. This study sheds new light on understanding the feature of genomic microsatellite DNA in P. forsythii, and lays a foundation for further studying the population genetic structure of P. forsythii by using microsatellite markers.
2019,38(5): 512-520 收稿日期:2019-01-09
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