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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.5

Association Analysis of Polymorphism of MC1R Gene with Recessive Yellow-Coated Color in Cavia porcellus
Author of the article:LAI Weining1, KANG Jianhong1, YU Feng1, HU Mingyue1, YAN Shouqing1, ZHU Wanju2*
Author's Workplace:1. College of Animal Science, Jilin University, Changchun 130062, China;
2. College of Veterinary Medicine, Jilin University, Changchun 130062, China
Key Words:Cavia porcellus; MC1R gene; coat color
Abstract:The recessive yellow-coated color in guinea pigs (Cavia porcellus) is determined by the e allele at extention locus encoding melanocortin receptor 1 (MC1R). Comparing to the wild-type guinea pigs, a 2 760 bp deletion including the entire coding region of MC1R gene was identified in the yellow-coated individuals by PCR amplification and sequencing. The results of PCR-based genotyping using 3 primers showed that among the 58 randomly selected wild-type guinea pigs, 36 were EE genotype and 22 were Ee, and all the 31 yellow-coated individuals were ee homozygous. Among the 15 test-crossing offsprings, all the 8 yellow-coated individuals were ee homozygous, while the remaining 7 wild-type individuals were Ee heterozygous, indicating the absolute correlation of the 2 760 bp deletion region of MC1R gene with the recessive yellow-coated color in C. porcellus. These results provide theoretical basis for further exploration of the function of MC1R gene in the genetic mechanism of mammalian coat color and molecular marker-assisted breeding in C. porcellus.
2019,38(5): 506-511 收稿日期:2019-04-01
刘迪文, 谢敏, 陈雁虹, 等. 2017. Zmu-1:DHP近交系豚鼠的培育及其分子遗传结构初步鉴定[J]. 中国实验动物学报, 25(1):90-96.
杨永升, 李宁, 邓学梅, 等. 2004. 黑素皮质素受体1——哺乳动物黑色素形成中的关键基因[J]. 遗传, 26(4):544-550.
赵珊珊, 肖宁, 李志鹏, 等. 2018. 马MC1R基因多态位点与毛色相关性分析[J]. 畜牧兽医学报, 49(8):1605-1616.
Bauer A, Kehl A, Jagannathan V, et al. 2018. A novel MLPH variant in dogs with coat colour dilution[J]. Animal Genetics, 49(1):94-97.
Druml T, Grilz-Seger G, Neuditschko M, et al. 2018. Novel insights into Sabino1 and splashed white coat color patterns in horses[J]. Animal Genetics, 49(3):249-253.
Everts RE, Rothuizen J, van Oost BA. 2000. Identification of a premature stop codon in the melanocyte-stimulating hormone receptor gene (MC1R) in labrador and golden retrievers with yellow coat colour[J]. Animal Genetics, 31(3):194-199.
Fontanesi L, Dall'olio S, Beretti F, et al. 2011. Coat colours in the Massese sheep breed are associated with mutations in the agouti signalling protein (ASIP) and melanocortin 1 receptor (MC1R) genes[J]. Animal, 5(1):8-17.
Fontanesi L, Tazzoli M, Beretti F, et al. 2006. Mutations in the melanocortin 1 receptor (MC1R) gene are associated with coat colours in the domestic rabbit (Oryctolagus cuniculus)[J]. Animal Genetics, 37(5):489-493.
García-Borrón JC, Sánchez-Laorden BL, Jiménez-Cervantes C. 2005. Melanocortin-1 receptor structure and functional regulation[J]. Pigment Cell Research, 18(6):393-410.
Ibsen HL. 1919. Tricolor inheritance. IV. The triple allelo-morphic series in guinea-pigs[J]. Genetics, 4(6):597-606.
Kijas JM, Wales R, Törnsten A, et al. 1998. Melanocortin receptor 1 (MC1R) mutations and coat color in pigs[J]. Genetics, 150(3):1177-1185.
Klungland H, Våge DI, Gomez-Raya L, et al. 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination[J]. Mammal Genome, 6(9):636-639.
Langevin M, Synkova H, Jancuskova T, et al. 2018. Merle phenotypes in dogs-SILV SINE insertions from Mc to Mh[J]. PLoS ONE, 13(9):e0198536. DOI:10.1371/journal.pone.0198536.
Marklund L, Moller MJ, Sandberg K, et al. 1996. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses[J]. Mammal Genome, 7(12):895-899.
Newton JM, Wilkie AL, He L, et al. 2000. Melanocortin 1 receptor variation in the domestic dog[J]. Mammal Genome, 11(1):24-30.
Rees JL. 2000. The melanocortin 1 receptor (MC1R):more than just red hair[J]. Pigment Cell Research, 13(3):135-140.
Robbins LS, Nadeau JH, Johnson KR, et al. 1993. Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function[J]. Cell, 72(6):827-834.
Thomas AJ, Erickson CA. 2008. The making of a melanocyte:the specification of melanoblasts from the neural crest[J]. Pigment Cell & Melanoma Research, 21(6):598-610.
Vidal O. 2018. Deleterious mutations of MC1R in guinea pig[J]. Animal Genetics, 49(5):498-499.
Wright S. 1927. The effects in combination of the major color-factors of the guinea pig[J]. Genetics, 12(6):530-569.
Yu F, Jiao S, Lai W, et al. 2018a. Conserved aspartate-to-glycine mutation in tyrosinase is associated with albino phenotype in domestic guinea pigs (Cavia porcellus)[J]. Animal Genetics, 49(4):354-355.
Yu F, Liu Z, Jiao S, et al. 2018b. A nonsense mutation in the FGF5 gene is associated with the long-coated phenotype in domestic guinea pigs (Cavia porcellus)[J]. Animal Genetics, 49(3):269. DOI:10.1111/age.12656.
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