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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.5

Association Analysis of Polymorphism of MC1R Gene with Recessive Yellow-Coated Color in Cavia porcellus
Author of the article:LAI Weining1, KANG Jianhong1, YU Feng1, HU Mingyue1, YAN Shouqing1, ZHU Wanju2*
Author's Workplace:1. College of Animal Science, Jilin University, Changchun 130062, China;
2. College of Veterinary Medicine, Jilin University, Changchun 130062, China
Key Words:Cavia porcellus; MC1R gene; coat color
Abstract:The recessive yellow-coated color in guinea pigs (Cavia porcellus) is determined by the e allele at extention locus encoding melanocortin receptor 1 (MC1R). Comparing to the wild-type guinea pigs, a 2 760 bp deletion including the entire coding region of MC1R gene was identified in the yellow-coated individuals by PCR amplification and sequencing. The results of PCR-based genotyping using 3 primers showed that among the 58 randomly selected wild-type guinea pigs, 36 were EE genotype and 22 were Ee, and all the 31 yellow-coated individuals were ee homozygous. Among the 15 test-crossing offsprings, all the 8 yellow-coated individuals were ee homozygous, while the remaining 7 wild-type individuals were Ee heterozygous, indicating the absolute correlation of the 2 760 bp deletion region of MC1R gene with the recessive yellow-coated color in C. porcellus. These results provide theoretical basis for further exploration of the function of MC1R gene in the genetic mechanism of mammalian coat color and molecular marker-assisted breeding in C. porcellus.
2019,38(5): 506-511 收稿日期:2019-04-01
分类号:S813.1
基金项目:国家自然科学基金项目(31601004);国家大学生创新创业训练计划项目(2018A8506)
作者简介:赖伟宁(1996-),女,本科生,E-mail:2390221455@qq.com
*通信作者:祝万菊,高级工程师,研究方向:动物机能学实验技术,E-mail:wjzhu@jlu.edu.cn
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