Abstract：Objective To explore the cause of contamination and provide a basis for ensuring and improving the quality of laboratory animals.
Methods The prevalence of
Staphylococcus aureus (SA) in SPF rats in an experimental animal farm, the environment, and the rearing staff were continuously monitored during 2011-2017 according to the National Standard (GB/T 14926.14-2001). All the isolated SA strains were classified by
Staphylococcus aureus protein A (SPA) typing and pulse field electrophoresis (PFGE) to trace the pollutant source.
Results A total of 16 SA strains were detected from 35 SPF rats with an isolation rate of 45.71%. Moreover, two SA strains were isolated from 18 samples of feeding environment and rearing staff, and among of which, one strain was detected in the air of the breeding room, and one strain was isolated from the nasopharyngeal swab of the rearing staff. The result of SPA typing showed that the obtained SA strains could be divided into 5 types. T2360 was the dominant type, which composed of strains from SPF rats in 2013 and 2017. Additionally, all the SA strains were divided into 5 types as determined by PFGE typing, and SA4 was found to be the main type. The PFGE pattern of SA isolated from the air of breeding room was identical to that from SPF rats in 2017.
Conclusion The contamination of SA in SPF rats in the experimental animal farm is closely related to the spreading of SA in the air of breeding room.
2019,38(4): 433-437 收稿日期：2018-11-14
分类号：Q95-33
基金项目：四川省科技厅省级科技计划项目（18PTDJ0027）
作者简介：黄伟峰(1987-),男,硕士研究生,主管技师,主要从事微生物检验
*通信作者:杨小蓉,主任技师,E-mail:yangyangxr@163.com
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