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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.4

Analysis of Thymosin THY3 Expression Patterns in Periplaneta americana
Author of the article:SUN Xiaohong1#, LI Ping2#, JING Jie1, GAO Jie1, YANG Zhen1, SHEN Yongmei3, ZHANG Xiuyue1, YUE Bisong1, MENG Yang1,4*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. College of Animal Science, Southwest University, Chongqing 402460, China;
3. Sichuan Key Laboratory for Medicinal American Cockroach, Chengdu 610031, China;
4. Museum of Natural History, Sichuan University, Chengdu 610065, China
Key Words:Periplaneta americana; thymosin; relative expression
Abstract:The thymosin gene of Periplaneta americana has 3 different spliceosomes including THY1, THY2, and THY3. THY3 possesses the largest number of structural domains. This study aims to figure out the expression difference of THY3 in different sexes, developmental stages and tissues of P. americana via real-time PCR. The effect of Escherichia coli-stimulation on THY3 expression was also analyzed. The results demonstrated that the expression of THY3 in adult P. americana was signifantly higher than any other stages. Female P. americana produced significantly higher amount of THY3 than male. Notably, fat had the highest THY3 expression level among the 5 tested tissues (fat, hemolymph, head, muscle and cuticle). Upon E. coli stimulation, the expression of THY3 in the hemolymph was significantly enhanced after 3 h, but increased in the fat after 12 h. These findings lay a solid foundation for further functional research of thymosin in P. americana.
2019,38(4): 415-419 收稿日期:2019-01-16
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