Determination of Peptide and Protein Diversity in the Venom of Macrothele yani
Author of the article:WANG Mengru1,2,3, ZHANG Xiuqing1,2,3, WANG Ying1,2,3, ZHOU Sitong1,2,3, YANG Zizhong1,2,3, LI Longxing3,4, YANG Zhibin1,2,3*
Author's Workplace：1. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R & D, Dali University, Dali, Yunnan Province 671000, China;
2. The National-Local Joint Engineering Laboratory for Entomoceutics, Dali, Yunnan Province 671000, China;
3. Southwest 2011 Collaborative Innovation Center for Development and Utilization of Medicinal Entomoceutics and Arachnoidea Resource, Dali University, Dali, Yunnan Province 671000, China;
4. Analyzing and Testing Center, Dali University, Dali, Yunnan Province 671000, China
Key Words：Macrothele yani; spider venom; peptides; proteins; diversity
Abstract：This study investigated the diversity of peptides and proteins in the venom of
Macrothele yani. In this experiment, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and ultra-performance liquid chromatography-electrospray-quadrupole-time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS) were used to separate and identify proteins and peptides in the venom, and the distribution of molecular mass was also analyzed. The results showed that the relative molecular mass of the proteins contained in the crude venom was mainly distributed between 17 kDa and 135 kDa, and there were 11 clearly separated electrophoretic bands with molecular mass ranged from 40 kDa to 120 kDa. Additionally, high-abundance protein bands were dispersed around 75 kDa, and the most abundant bands were found over 75 kDa. Furthermore, more than 50 chromatographic peaks were separated by UPLC and 121 constituents were identified by MS analysis. 21% peptides distributed in the range of 500 Da to 2 000 Da while 76% were distributed in the range of 3 000 Da to 5 000 Da showing a bimodal distribution. As the most abundant peptides content in the crude venom of
M. yani, the 76% peptides were eluted during the retention time of 35 min to 60 min. The results show that the venom of
M. yani contains abundant peptides and proteins. However, this study lays a foundation for the material basic research and drug development of the toxin in the future.
2019,38(4): 408-414 收稿日期：2019-01-21
分类号：Q51;Q95
基金项目：国家地方联合工程研究中心创新能力建设项目（云发改高技[2015]395）；云南省科技创新平台建设项目（2015DG030）；云南省应用基础研究重点项目（2016FA012）；云南省应用基础研究青年项目（2016FD074）
作者简介：王梦如(1992-),硕士研究生,从事昆虫和动物毒素方面的研究,E-mail:15947971269@163.com
*通信作者:杨志斌,E-mail:79204431@163.com
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