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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.4

Determination of Peptide and Protein Diversity in the Venom of Macrothele yani
Author of the article:WANG Mengru1,2,3, ZHANG Xiuqing1,2,3, WANG Ying1,2,3, ZHOU Sitong1,2,3, YANG Zizhong1,2,3, LI Longxing3,4, YANG Zhibin1,2,3*
Author's Workplace:1. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R & D, Dali University, Dali, Yunnan Province 671000, China;
2. The National-Local Joint Engineering Laboratory for Entomoceutics, Dali, Yunnan Province 671000, China;
3. Southwest 2011 Collaborative Innovation Center for Development and Utilization of Medicinal Entomoceutics and Arachnoidea Resource, Dali University, Dali, Yunnan Province 671000, China;
4. Analyzing and Testing Center, Dali University, Dali, Yunnan Province 671000, China
Key Words:Macrothele yani; spider venom; peptides; proteins; diversity
Abstract:This study investigated the diversity of peptides and proteins in the venom of Macrothele yani. In this experiment, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and ultra-performance liquid chromatography-electrospray-quadrupole-time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS) were used to separate and identify proteins and peptides in the venom, and the distribution of molecular mass was also analyzed. The results showed that the relative molecular mass of the proteins contained in the crude venom was mainly distributed between 17 kDa and 135 kDa, and there were 11 clearly separated electrophoretic bands with molecular mass ranged from 40 kDa to 120 kDa. Additionally, high-abundance protein bands were dispersed around 75 kDa, and the most abundant bands were found over 75 kDa. Furthermore, more than 50 chromatographic peaks were separated by UPLC and 121 constituents were identified by MS analysis. 21% peptides distributed in the range of 500 Da to 2 000 Da while 76% were distributed in the range of 3 000 Da to 5 000 Da showing a bimodal distribution. As the most abundant peptides content in the crude venom of M. yani, the 76% peptides were eluted during the retention time of 35 min to 60 min. The results show that the venom of M. yani contains abundant peptides and proteins. However, this study lays a foundation for the material basic research and drug development of the toxin in the future.
2019,38(4): 408-414 收稿日期:2019-01-21
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