Study on Tissue Repair Mechanism of Sika Deer Antler Velvet Skins Based on Transcriptomics
Abstract：We aimed to study the gene expression pattern of the velvet skin of sika deer (
Cervus nippon hortulorum) antler at different growth stages, and to provide theoretical basis for the growth mechanism of the antler as well as the clinical treatments of tissue repair. Illumina RNA sequencing and bioinformatic tools were conducted to analyze the gene expression pattern of velvet skins at rapid growth and ossification stages. Finally, 45 422 254 and 44 530 430 clean reads with Q20 values of 98.50 and 98.39 were generated from the rapid growth and the ossification stages, respectively. A total of 44 352 and 43 194 Unigenes with average lengths of 977 nt and 1 135 nt were obtained by using Trinity software. We identified 7 growth factors, 25 transcription factors and 6 collagens by BLAST against the protein database. Among these genes, a series of genes (including Pdgfa, Tgfβ3, Egfl7, Vegfb, Sox4, Sox12, Scx, E4f1, Tcf4 and Elf4) which play pivotal roles in regulating tissue repair were identified. Our current study provides a data basis for the mechanistic studies on antler growth and tissue repair.
2019,38(4): 394-401 收稿日期：2019-01-05
分类号：Q95-33;Q786
基金项目：国家重点研发计划项目（2018YFC1706605）；吉林省教育厅“十三五”科学技术研究项目（JJKH20181269KJ；JJKH20181272KJ；JJKH20170721KJ）；吉林省科技厅优秀青年人才基金项目（20170520044JH）
作者简介：杨永刚(1976-),男,博士,助理研究员,研究方向为鹿茸生长机制及健康产品开发,E-mail:ccyyg1976@sina.com
*通信作者:幺宝金,E-mail:ybj19830928@sina.com
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