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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.4

Study on Tissue Repair Mechanism of Sika Deer Antler Velvet Skins Based on Transcriptomics
Author of the article:YANG Yonggang1, ZHANG Mei2, HU Yaozhong3, ZHAO Yu3, ZHAO Daqing3, YAO Baojin3*
Author's Workplace:1. Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130117, China;
2. Innovation Practice Center, Changchun University of Chinese Medicine, Changchun 130117, China;
3. Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
Key Words:Cervus nippon; growth center; velvet skin; transcriptomics; tissue repair
Abstract:We aimed to study the gene expression pattern of the velvet skin of sika deer (Cervus nippon hortulorum) antler at different growth stages, and to provide theoretical basis for the growth mechanism of the antler as well as the clinical treatments of tissue repair. Illumina RNA sequencing and bioinformatic tools were conducted to analyze the gene expression pattern of velvet skins at rapid growth and ossification stages. Finally, 45 422 254 and 44 530 430 clean reads with Q20 values of 98.50 and 98.39 were generated from the rapid growth and the ossification stages, respectively. A total of 44 352 and 43 194 Unigenes with average lengths of 977 nt and 1 135 nt were obtained by using Trinity software. We identified 7 growth factors, 25 transcription factors and 6 collagens by BLAST against the protein database. Among these genes, a series of genes (including Pdgfa, Tgfβ3, Egfl7, Vegfb, Sox4, Sox12, Scx, E4f1, Tcf4 and Elf4) which play pivotal roles in regulating tissue repair were identified. Our current study provides a data basis for the mechanistic studies on antler growth and tissue repair.
2019,38(4): 394-401 收稿日期:2019-01-05
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