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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.3

Discovery of Stichophanes ningshaanensis in Dabashan National Nature Reserve, Chengkou County, Chongqing City, with Intraspecific Genetic Divergence of the Species
Author of the article:CHEN Zening1, HE Jie2, WU Jun3, JIANG Jianping1, DING Li1*, WANG Bin1*
Author's Workplace:1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
2. Southwest Petroleum University, Chengdu 610500, China;
3. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
Key Words:Stichophanes ningshaanensis; Dabashan National Nature Reserve; population divergence; phylogenetic; morphology
Abstract:Stichophanes ningshaanensis Yuan, 1983, which belonging to the monotypic genus Stichophanes of the family Colubridae, is endemic to China. To present, this species was only known to be distributed in Huoditang, Ningshaan county, Shaanxi province, Shennongjia National Nature Reserve, Hubei province, and Wulipo National Nature Reserve, Chongqing city. Few studies have been focused on this species due to the lack of wild specimens. On July 8, 2017, one female specimen presented similar morphological features with S. ningshaanensis was collected from Dabashan National Nature Reserve (Chengkou county, Chongqing city). Based on sequence data of mitochondrial cyt b gene and nuclear gene cmos, the results of phylogenetic analyses suggested that this specimen was clustered together with the specimens from the other 2 known populations, which were clustered into a monophyly corresponding to S. ningshaanensis. Genetic distance between the 3 populations of the species was 0.001-0.009, which was significantly smaller than that between the species and its related species (0.174-0.292). Moreover, there was considerable divergence among different populations of this species. On morphology, the specimen from Dabashan had only 5 supralabials on both sides and 61 pairs of subcaudals, while the others had 6 supralabials and 64-73 pairs of subcaudals. On cyt b gene, the 3 populations occupied distinct haplotypes. There was a mutation between the haplotypes from Chengkou county and Shennongjia, Hubei province, while 9 mutations were detected between haplotypes from Chengkou county and Huoditang, Ningshaan county. However, phylogenetic analyses suggested that Shennongjia population was closer to Huoditang population. This study provides a basis for future investigations on the distribution and systematics of Stichophanes species.
2019,38(3): 249-255 收稿日期:2018-12-04
分类号:Q959.6;Q953
基金项目:国家重点研发计划项目(2016YFC1200705);生物多样性保护重大工程-重庆市城口县两栖爬行动物本底调查与评估项目;国家自然科学基金项目(31471964,31201702);中国科学院生物多样性监测研究网络项目(Sino-BON)
作者简介:陈泽柠(1989-),男,博士研究生,研究方向:爬行动物系统学,E-mail:chenzeningwy@163.com
*通信作者:丁利,E-mail:dingli@cib.ac.cn;王斌,E-mail:wangbin@cib.ac.cn
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