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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.1

Comparison of Gut Microbiome in Macaca thibetana Between Mount Emei and Mount Huangshan
Author of the article:ZHAI Zihao1, SONG Yang1, WANG Junyin2, ZHANG Kejun2, SUN Binghua3*, LI Jing1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Mount Emei Scenic Area Management Committee, Mount Emei Biodiversity Conservation Institute, Emeishan, Sichuan Province 614200, China;
3. School of Resources and Environmental Engineering, Anhui University, International Joint Research Center for Biodiversity and Macaque Behavioral Ecology in Huangshan, Hefei 230039, China
Key Words:Macaca thibetana; Mount Emei; Mount Huangshan; gut microbiome; diversity; 16S rRNA gene
Abstract:Mount Emei (EM) and Mount Huangshan (HS) are both famous for the Macaca thibetana ecotourism in China. Study on the composition difference of gut microbiome in M. thibetana from these 2 places will improve our understanding of the effects of habitats and tourism managements on wild primates. In this study, 16S rRNA gene was used as molecular marker to explore the gut microbiome of M. t. thibetana in EM, and M. t. huangshanensis in HS. The result showed that a large number of operational taxonomic units were shared by the gut microbiome of M. thibetana from EM and HS. Remarkably, significant differences were detected in community composition and microbial diversity. The dominant phylum microbial communities in EM were Firmicutes (69.04%±11.81%), Bacteroidetes (21.59%±10.05%) and Actinobacteria (2.73%±2.17%). By contrast, Firmicutes (46.34%±8.15%), Bacteroidetes (36.75%±6.38%) and Proteobacteria (14.91%±8.06%) were the dominant in HS. The most abundant genus in EM was Oscillospira (23.49%±16.63%) and that in HS was Prevotella (36.35%±9.15%). In terms of the community diversity, the α diversity index in HS was significantly lower than EM, and their microbial community structures also had a significant difference. PICRUSt analysis revealed that the enriched KEGG pathways of gut microbiome in EM were lipid metabolism and xenobiotics biodegradation and metabolism. Differently, glycan biosynthesis and metabolism, etc were enriched in HS. In addition, several infectious pathogenic bacteria were found in the gut of EM, and this was probably related to the ecotourism in EM.
2019,38(1): 1-10 收稿日期:2018-06-12
分类号:Q959.848
基金项目:国家自然科学基金项目(31530068,31770415)
作者简介:翟子豪,男,主要研究藏酋猴肠道微生物,E-mail:zhaizihaoace@yeah.net
*通信作者:孙丙华,E-mail:binghuasun00@126.com;李静,E-mail:ljtjf@126.com
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