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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.6

Polyethyleneimine Enhances in situ Gene Delivery and Expression in Skeletal Muscles of BALB/c Mice in the Pluronic-Mediated System
Author of the article:HE Yutong, SUN Zhe, WANG Gang*
Author's Workplace:Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, China
Key Words:BALB/c mice; skeletal muscle gene delivery; polyethyleneimine; Pluronic L64
Abstract:Objective To correctly introduce cationic polyethyleneimine (PEI) into the Pluronic L64-mediated gene delivery system of mice skeletal muscle to enhance the efficiency of gene expression.Methods In this study, different PEI samples were transferred into mice tibialis anterior muscles via intramuscular injection. The saline, plasmid DNA (pDNA) and Pluronic L64/pDNA (L/D) were used as control groups, and the mixtures of PEI/pDNA with different N/P ratios (L/P/D-0.5, L/P/D-3, and L/P/D-10) and Pluronic L64 were used as experimental groups. Muscle tissues were taken out at 7 days after injection, and then the report gene expression of β-galactosidase and luciferase and the potential pathological changes of muscle tissues were measured. In addition, the in vivo acute toxicity experiment was further determined by injecting L/P/D-0.5 through tail vein. Finally, the particle size, zeta potential and morphology of PEI/pDNA complexes in L/P/D-0.5 system were detected by dynamic light scattering instrument and atomic force microscope (AFM).Results 1) The L/P/D-0.5 group showed the highest report gene expression; As N/P ratio increasing, the report gene expression could hardly be detected. 2) The result of biocompatibility assessment showed that no tissue lesion was observed in L/P/D-0.5 group irrespective of muscular injection or intravenous injection, while other ratios of L/P/D showed histologic lesions with varying degrees. 3) PEI/pDNA complexes were spherical shape with nanometer size, and this might contribute to the process of gene delivery in skeletal muscle-based system, although pDNA was not fully compressed in L/P/D-0.5 group.Conclusion The L/P/D-0.5 system can safely and effectively enhance the expression of exogenous genes in skeletal muscle.
2018,37(6): 620-627 收稿日期:2018-03-14
分类号:Q95-336
基金项目:国家自然科学基金项目(31370972)
作者简介:何雨桐(1992—),女,硕士研究生,研究方向:生物化学与分子生物学,E-mail:kelseyhe@foxmail.com
*通信作者:王刚,研究员,研究方向:靶向基因传递、基因治疗,E-mail:wgang@scu.edu.cn
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