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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.1

Ultrastructural Observation of the Sperm of Sinibotia reevesae and Effect of Na+, K+ and Ca2+ on its Motility
Author of the article:WANG Yongming1, CHEN Yu1, HU Yu2, XIE Biwen1, YUE Xingjian1, WANG Fang1, QI Zemin1, WANG Yu1
Author's Workplace:1. College of Life Sciences, Neijiang Normal University, Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Neijiang, Sichuan Province 641112, China;
2. Southwest University, Key Laboratory of Aquatic Organism Reproduction and Developement, Chongqing 400715, China
Key Words: Sinibotia reevesae; sperm; ultrastructure; sperm motility
Abstract:In order to study the ultrastructure of the sperm of Sinibotia reevesae and the effect of Na+, K+ and Ca2+ on its motility, JSM 6510LV scanning electron microscope, H-7500 transmission electron microscope and Motic-BA210 digital microscope were used, respectively. The results showed that the head of S. reevesae sperm was round without acrosome. There was an implantation fossa in the end of the nucleus and about 1/6 of the length of nuclei. The mid-piece consisted of centriolar complex and sleeves. The centriolar complex was divided into proximal centriole and matrix and arranged in an "L" type. Sleeves were found on both sides of axon, and one side was long and narrow, whereas the other side was hypertrophy. The tail was a typical "9+2" type consisting of axoneme. In conclusion, the best activation medium of S. reevesae sperm should be made by deionized water with NaCl 75 mmol·L-1, KCl 0.5 mmol·L-1 and CaCl2 5 mmol·L-1. It suggested that the optimal activation medium should be selected while using artificial insemination.
2016,(1): 38-43 收稿日期:2015-06-18
DOI:10.11984/j.issn.1000-7083.20150213
分类号:Q959.4
基金项目:四川省教育厅重大培育项目(09ZZ012); 四川省省属高校科研创新团队项目(KYTD201009); 四川省科技支撑项目(2011NZ0075)
作者简介:王永明(1985-), 男, 讲师, 研究方向为鱼类形态及发育生物学
*通讯作者:谢碧文, 教授, E-mail:xiebw6873@163.com
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