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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.1

Diversity Analysis and Antimicrobial Susceptibility Test of Lactic Acid Bacteria and Enterobacteria that Isolated from Forest Musk Deer Intestine
Author of the article:MA Bingcun1, CHENG Jianguo2, LUO Yan1*, LUO Xi1,3, ZHOU Xin1,3, GAO Xuehua1, WANG Peng1
Author's Workplace:1. Lab of Microbiology, Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan, Sichuan Province 611830, China;
2. Sichuan Institute of Musk Deer Breeding, Dujiangyan, Sichuan Province 611830, China;
3. College of Veterinary Medicine, Ya'an Campus, Sichuan Agricultural University, Ya'an, Sichuan Province 625000, China
Key Words:lactic acid bacteria; enterobacteria; pulsed filed gel electrophoresis; antimicrobial susceptibility; forest musk deer
Abstract:To investigate the phenotypic and genotypic diversity of bacteria in forest musk deer intestine, a total of 38 isolates, including 22 enterobacteria and 16 lactic acid bacteria (LAB), were isolated and identified by physiological and molecular methods. Pulsed filed gel electrophoresis (PFGE) was conducted to analysis the genotypic diversity, and antimicrobial susceptibility test was carried out to evaluate the antibiotic resistance of the isolates. Finally, a total of 11 species, Escherichia coli, Enterobacter cloacae, E. amnigenus, Klebsiella pneumoniae, Enterococcus mundtii, E. durans, Leuconostoc fallax, Lactobacillus plantarum, L. acidipiscis, E. faecium and Weissella cibaria, were identified. E. coli was the predominant species (n=11, 28.9%), while Enterococcus is the most abundant LAB species (n=10, 26.3%). The results of PFGE revealed seven different patterns of enterobacteria and nine of LAB. The antimicrobial susceptibility results indicated that most of the inherent bacterial species were susceptible to the most commonly used antibiotics. This was the first time to analyze the phenotypic and genotypic diversity as well as the antimicrobial susceptibility of culturable bacteria in intestine of forest musk deer.
2015,34(1): 29-40 收稿日期:2014-6-24
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