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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.1

Characteristics of Microsatellites in Arborophila rufipectus Genome Sequences Using 454 GS FLX
Author of the article:HUANG Jie1, ZHOU Yu1, LIU Yuzhi1, ZHU Dan1, SONG Xuhao1, CHEN Benping2, ZHANG Xiuyue1, YUE Bisong1*
Author's Workplace:1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610064, China;
2. Laojunshan National Nature Reserve Management Bureau, Pingshan, Sichuan Province 645350, China
Key Words:Arborophila rufipectus; genome; microsatellite; distribution
Abstract:The Sichuan hill partridge (Arborophila rufipectus) is a critically endangered species endemic to China. The entire genome of A. rufipectus was sequenced using Roche's 454 GS FLX platform. Microsatellites were identified by screening the genome sequences in silico mining and a large number of different kinds of repeat motif types of perfect microsatellite sequences were discovered. A total of 335 263 simple sequence repeats (SSRs) with 1~6 bp nucleotide motifs were identified. Moreover, A, AC, AAC, AAAC, AAACA, and AGGGTT were the most frequent repeat copy units of the six motif types, respectively. In the different repeat types of the microsatellites, mononucleotide (59.03%) was the most common repeat unit followed by the tetra (13.59%), tri (8.39%), hexa (7.55%), di (6.49%) and pentanucleotides (4.95%). In the A. rufipectus genome, A, AGGGTT, AAAC, AC, AAAT, AAC, C, AG, AAAG, AAACA, AAT, AGG, AT, AGC, AAGG, and CCG were the 16 predominant repeat types. This study provided significant genome information of A. rufipectus, which will contribute to further understanding of this species. The resources that we have developed will certainly facilitate the isolation of large numbers of high quality microsatellite markers for genetic diversity studies within species in the future.
2015,34(1): 8-14 收稿日期:2014-6-16
DOI:10.3969/j.issn.1000-7083.2015.01.002
分类号:Q959.3;R857.3
基金项目:国家自然科学基金(31172118)
作者简介:黄杰(1987-),女,博士研究生
*通讯作者:岳碧松,E-mail:bsyue@scu.edu.cn
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