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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.5

Protection of β-Ecdysone on Thymic Involution in Mice
Author of the article:FENG Cheng, CHEN Zhijuan, LUO Yu, WU Yahui, GAO Ping*
Author's Workplace:(Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, School of Life Sciences, Sichuan University, Chengdu 610065, China)
Key Words: β-ecdysone; thymus; involution; apoptosis
Abstract:Objective  To investigate the protection of β-Ecdysone (Ecd) on thymic involution in mice, the model was established by intraperitoneal injection of dexamethasone (Dex). Methods  Mice were randomly divided into five groups: the normal control group (control group), the thymic involution model group (model group) and three experimental groups which were given three dosages of Ecd (50 mg/kg, 75 mg/kg, 100 mg/kg) for seven days respectively. After 15 hours after intraperitoneal injection of Dex, thymuses were isolated and then the thymic index was computed. The histological changes of thymus were determined by HE staining. The expression levels of Bcl-2 and Bax in thymus were detected by immunohistochemistry. Conclusion  The thymic index and the expression of Bcl-2 were significantly heightened, while the expression of Bcl-2 and the ratio of Bcl-2/Bax were enhanced. The results confirmed that Ecd can protect thymus from Dex-induced involution by protecting the structure of thymic tissues, inhibiting thymocyte apoptosis and improving survival rate of thymocytes.
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