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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Preliminary Research of MicroRNAs in American Cockroach (Periplaneta Americana)
Author of the article:BAO Z, YANG M, ZHANG X, YUE B,FAn Z
Author's Workplace:Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences,Sichuan University, Sichuan Engineering Research Center for Medicinal Animals Sichuan Key Laboratory for Medicinal American Cockroach,
Key Words:Periplaneta americana; microRNA; differential expression
Abstract:American cockroach (Periplaneta Americana) is known as one of worldwide pests. Meanwhile, its extracts are valuable for medical use. In this study, the reared cockroaches were carried out sequencing of small RNAs. Total of 12,155,616 and 9,847,263 sequences were obtained from male and female groups, respectively. The length of sequences ranged from 18nt to 23nt revealing a bimodal distribution at 22nt and 29nt. Annotation of these clean reads were matched to database Rfam and NCBI by tool blastn. Then 57 known miRNAs and 152 novel miRNAs were identified from the male group, and 53 known miRNAs and 94 novel miRNAs were identified from the female group. Differential expression analysis indicated that only one miRNA, the miR-750 was significantly higher expressed in female P. americana than male P. americana. This is the first study of the composition and function of microRNAs in P. americana at genomic level, which has laid a foundation for the follow-up study for the P. americana.
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