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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Characteristics of Microsatellites in Phrynocephalus axillaris Genome Sequences Using Roche 454 GS FLX
Author of the article:Qi SONG, Jinlong LIU, Xianguang GUO
Author's Workplace:Chengdu Institute of Biology, Chinese Academy of Sciences
Key Words:Phrynocephalus axillaris; genome; microsatellite; distribution
Abstract:Yarkand toad-headed agama (Phrynocephalus axillaris), a small reptile endemic to China, is widely distributed in Tarim Basin, Turpan-Hami Basin, Xinjiang Uygur Autonomous Region and Dunhuang Basin, Gansu province. The entire genome of P. axillaris was sequenced using Roche 454 GS FLX platform, and 91 190 high quality sequences were obtained. A total of 29 890 simple sequence repeats (SSRs) with 1~6 bp nucleotide motifs were identified by using Krait software. Moreover, AC, ATC, AAAT, AAAAT, and AATCCC were the most frequent repeat copy units of the two to six motif types, respectively. In the different repeat types of the microsatellites, mononucleotide (48.95%) was the most common repeat unit followed by the di- (28.60%), tetra- (10.73%), tri- (10.48%), penta- (0.92%) and hexanucleotides (0.32%). In the genome of P. axillaris, there were eleven predomiant repeat types, which included C, A, AC, AG, AAAT, ATC, AT, AAT, ATAG, AGG and AAC. This study has deepened the understanding the genome of P. axillaris, and will provide data support for development and screening of a large number of high quality microsatellite markers. In addition, this study has laid a foundation for revealing population genetic structure and phylogeographic pattern of this lizard using microsatellite markers.
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