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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Molecular Cloning and Tissue Expression of Chicken Natriuretic Peptide Receptors B Gene
Author of the article:LI Zhengyang, ZHANG Jiannan, CHEN Junan, LI Juan, WANG Yajun
Author's Workplace:Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University
Key Words:NPR-B;Gene cloning;Functional analysis;Tissue expression;Physiological function
Abstract:As a selective receptor of C-type natriuretic peptide (CNP), natriuretic peptide receptor B (NPR-B) regulates various physiological processes, including pituitary hormone secretion, cardiovascular stability, development of nervous system, cell proliferation and bone growth. In this study, the full-length cDNA of chicken NPR-B gene was reported and its tissue expression in adult chickens was detected by qPCR and RNA-seq analyses. The results showed that chicken NPR-B gene has a full length of 3189 bp and encodes a receptor of 1062 amino acids, which shows high amino acid sequence identitiy with that of human (Homo sapiens,79%), rats (Rattus rattus, 78%), Xenopus laevis (73%) and zebrafish (Danio rerio, 67%). Quantitative real-time PCR were used to investigate the expression of NPR-B in chicken tissues. It was found that NPR-B is highly expressed in the heart, muscle, midbrain and pituitary. The results of this study laid the foundation for explaining the physiological functions of CNP and NPR-B in chickens.
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