刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/四川省野生动植物保护协会/四川大学/成都大熊猫繁育研究基金会
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610065
电话:028-85410485; 15881112385
传真:
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2022年第41卷第4期

基于mtDNA D-loop部分序列的软刺裸裂尻鱼遗传多样性分析
Genetic Diversity of Schizopygopsis malacanthus Based on Partial Sequence of mtDNA D?loop
杨朝杰1,2, 高强1, 刘丹1, 聂苗苗1, 王发艳1, 李柯懋3,赵宏4,张存芳1*,祁得林1*
点击:105次 下载:4次
DOI:10.11984/j.issn.1000-7083.20210445
作者单位:1.青海大学省部共建三江源生态与高原农牧业国家重点实验室,西宁 810016
2.青海大学农牧学院,西宁 810016
3.青海省渔业技术推广中心青海省渔业环境监测中心,西宁 810012
4.青海多美生态环保科技有限公司,西宁 810003
中文关键字:软刺裸裂尻鱼;D?loop;遗传多样性;种群遗传结构
英文关键字:D?loop;genetic diversity;population genetic structure;
中文摘要:软刺裸裂尻鱼Schizopygopsis malacanthus是青藏高原特有鱼类。本研究测定了青海省玉树藏族自治州3个软刺裸裂尻鱼野生群体(隆宝滩国家级自然保护区、巴塘河上游、巴塘河下游)共72尾的mtDNA D-loop的部分序列(610 bp),分析了其遗传多样性和种群遗传结构,旨在为玉树软刺裸裂尻鱼野生资源保护和合理开发提供理论依据。共发现29个多态位点,定义了16个单倍型。3个种群的单倍型多样性为0.438±0.121~0.676±0.076,核苷酸多样性为0.003 4±0.000 5~0.008 6±0.001 3。群体遗传分化指数统计检验表明,两两群体之间的差异均极显著。基因流分析结果表明,巴塘河上、下游种群之间的基因流水平较高,个体交流频繁。AMOVA分析结果显示,79.49%的遗传变异来自于种群间,20.51%的遗传变异来自于种群内,群体间遗传分化极显著。系统发育树和单倍型进化网络结果显示,3个种群的单倍型按照隆宝滩国家级自然保护区和巴塘河水系形成2大类群。中性检验结果表明,软刺裸裂尻鱼近期未出现显著的种群扩张事件。建议将分布在隆宝滩国家级自然保护区和巴塘河水系的软刺裸裂尻鱼作为2个进化单元分开管理。
英文摘要:Schizopygopsis malacanthus is a fish species endemic to the Qinghai‑Tibet Plateau. In order to analyze the genetic diversity and population genetic structure of S. malacanthus, and to provide a theoretical basis for conservation management of the wild resources. The partial sequence (610 bp) of the mtDNA D‑loop of 72 individuals from 3 wild populations of S. malacanthus (Longbaotan National Nature Reserve, upper Batang River and lower Batang River) in Yushu Tibetan Autonomous Prefecture, Qinghai Province were determined. The results showed that a total of 29 polymorphic sites were found, and 16 haplotypes were defined. The haplotype diversity of the 3 populations ranged from 0.438±0.121 to 0.676±0.076, and the nucleotide diversity ranged from 0.003 4±0.000 5 to 0.008 6±0.001 3. Fst value among the 3 populations were extremely significant. The level of gene flow between the upstream and downstream of Batang River was higher, and the individuals’ exchanges were frequent. AMOVA analysis revealed that 79.49% of genetic variation occurred among populations, and 20.51% occurred within populations. The genetic differentiation between populations was extremely significant. The results of phylogenetic analysis and haplotype evolution network showed that the haplotypes of the 3 populations formed 2 major clusters. The results of the neutral test showed that there was no significant population expansion event recently. This study indicates that the populations of S. malacanthus from the Longbaotan National Nature Reserve and the Batang River should be managed and conserved separately as 2 evolutionary units in the future conservation plans.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准连续出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/四川省野生动植物保护协会/四川大学/成都大熊猫繁育研究基金会
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2022四川动物》编辑部 蜀ICP备08107403号-3
您是本站第12190827名访问者

川公网安备 51010702000173号