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基于4个线粒体基因的不同海拔高原林蛙群体遗传多样性研究
Genetic Diversity of Rana kukunoris Populations from Different Elevations Based on Four Mitochondrial Genes
郑海钱, 徐志旺, 徐康宁, 李卉, 李树然, 张永普*
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DOI:10.11984/j.issn.1000-7083.20210465
作者单位:温州大学生命与环境科学学院,浙江温州325035
中文关键字:线粒体基因;青藏高原;两栖动物;海拔;遗传多样性
英文关键字:mitochondrial genes;Qinghai?Tibet Plateau;amphibian;elevation;genetic diversity
中文摘要:高原林蛙Rana kukunoris是中国特有的两栖动物,分布于青藏高原海拔2 000~4 400 m的湿润环境。为探究青藏高原的高原林蛙遗传多样性,将4个线粒体基因(COⅠ、cyt b、12S rRNA和16S rRNA)序列合并成联合数据集,对采自青海省4个海拔梯度(2 000 m、2 600 m、3 200 m和3 800 m)的100只高原林蛙个体进行测序分析。结果表明,2 000 m和2 600 m的高原林蛙群体的多态位点、单倍型多样性、核苷酸多样性和平均核苷酸差异数高于3 200 m和3 800 m群体;4个群体均出现了高单倍型多样性、低核苷酸多样性的结果。高原林蛙遗传分化主要发生在群体间,中性检验和错配分析表明,高原林蛙群体可能没有经历扩张事件。cyt b基因在2 600 m、3 200 m和3 800 m群体都检测到了正选择位点。综上所述,2 000 m和2 600 m的高原林蛙群体遗传多样性高于3 200 m和3 800 m群体,4个海拔群体的遗传分化程度较高;cyt b基因可能对高原林蛙适应高海拔环境起到了积极作用。本研究可为理解高原林蛙适应高原环境及其保护提供参考。
英文摘要:Rana kukunoris is an amphibian species endemically distributed in the humid environment of the Qinghai-Tibet Plateau at an elevation of 2 000-4 400 m. This study collected 100 individuals of R. kukunoris from 4 elevations (2 000 m, 2 600 m, 3 200 m and 3 800 m). The mitochondrial genes including COⅠcyt b12S rRNA and 16S rRNA of the individuals were obtained and combined to analyze the genetic diversity. The results showed that the number of polymorphic sites, haplotype diversity, and nucleotide diversity, and the average number of nucleotide differences of R. kukunoris population from 2 000 m and 2 600 m were higher than those from 3 200 m and 3 800 m. All the 4 populations showed high haplotype diversity and low nucleotide diversity. In addition, genetic differentiation mainly occurred between populations, and the results of neutral test and mismatch analysis indicated that R. kukunoris might not experience population expansion events. Positive selection sites were detected in the cyt b gene in the populations from 2 600 m, 3 200 m, and 3 800 m. In summary, the genetic diversity of R. kukunoris from 2 000 m and 2 600 m populations are higher than those from 3 200 m and 3 800 m populations, and there is a high degree of genetic differentiation among the 4 populations. Moreover, cyt b gene may play an important role in the adaptation of R. kukunoris to high‑elevation environments. These results contribute to understanding the high‑elevation adaptation of R. kukunoris.
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国内统一连续出版物号:51-1193/Q |国际标准连续出版物号:1000-7083
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