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双委夜蛾感觉神经元膜蛋白编码基因鉴定及组织表达谱分析
Cloning and Expression Pattern of the Sensory Neuron Membrane Protein-Encoding Genes of Athetis dissimilis
宋智煜1, 陈双臣1, 白小军2, 董钧锋1, 宋月芹1*
点击:117次 下载:17次
DOI:10.11984/j.issn.1000-7083.20200424
作者单位:1. 河南科技大学林学院, 河南洛阳 471000;
2. 宜阳县林业技术指导站, 河南洛阳 471600
中文关键字:双委夜蛾;感觉神经元膜蛋白;克隆;序列分析;表达谱
英文关键字:Athetis dissimilis; sensory neuron membrane proteins; cloning; sequence analysis; expression profile
中文摘要:昆虫感觉神经元膜蛋白(SNMP)是至关重要的次要嗅觉蛋白,能够作为信息素识别的标记。本研究利用荧光定量PCR方法首次克隆和鉴定了双委夜蛾Athetis dissimilis的2个SNMP基因,并命名为AdisSNMP1AdisSNMP2。序列分析表明,AdisSNMP1编码区开放阅读框长1 572 bp,编码523个氨基酸;而AdisSNMP2编码区开放阅读框长1 563 bp,编码520个氨基酸。这2个基因编码蛋白质为酸性,分子量约58 kD。同源性分析发现,SNMP1和AdisSNMP2之间的序列一致性极低。进化树结果也表明同目昆虫SNMP基因进化关系最近。荧光定量PCR结果显示,AdisSNMP1主要在雌雄触角中表达,而AdisSNMP2在非触角组织中也有表达。此结果为今后对双委夜蛾SNMPs基因功能的研究提供了参考。
英文摘要:Sensory neuron membrane proteins (SNMPs) in insects are critical peripheral olfactory proteins and act as markers for pheromone detection. In this study, two SNMP genes of Athetis dissimilis, named AdisSNMP1 and AdisSNMP2, were cloned and identified for the first time by quantitative real-time PCR. Sequence analysis showed that the encoding area of AdisSNMP1 gene is 1 572 bp in length, and the encoding area of AdisSNMP2 gene is 1 563 bp in length. Both genes encoding proteins are acidic, and the molecular weights are about 58 kD. Homology analysis showed that the consistency of SNMPgenes among the insects from the same order was high, the consistency of them with the species from different order was low, and the sequence consistency between SNMP1 and AdisSNMP2 was extremely low. The results of phylogenetic analysis also showed that the evolutionary relationship of SNMP genes of A. dissimilis with the insects from the same order were close. Quantitative real-time PCR showed that AdisSNMP1 was mainly expressed in the antennae of female and male individuals, whereas AdisSNMP2 was also expressed in other tissues. The study provides useful reference for further studies on the function of A. dissimilis SNMP genes.
2021,40(2): 121-129 收稿日期:2020-11-09
分类号:Q966
基金项目:国家自然科学基金项目(31701788);河南省科技攻关计划(农业领域)项目(202102110069;182102110182)
作者简介:宋智煜(1996-),研究生,主要研究领域为生物与分子生物学,E-mail:lylhsys@126.com
*通信作者:宋月芹,E-mail:songyueqin6@haust.edu.cn
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