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产双羔彭波半细毛羊发情期卵巢基因表达差异研究
Expression Differences of Ovarian Genes of Pengbo Semi Fine Wool Sheep Producing Twin Lambs at Estrus
张立1*, 普布次仁1, 胡亚东2, 扎西1, 洛桑催成1, 次仁曲珍1, 德庆卓嘎1
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DOI:10.11984/j.issn.1000-7083.20190341
作者单位:1. 西藏自治区农牧科学院畜牧兽医研究所, 拉萨 850009;
2. 中国科学院成都生物研究所, 成都 610041
中文关键字:彭波半细毛羊;产羔数性状;卵巢发育;转录组测序;差异表达基因
英文关键字:Pengbo semi fine wool sheep; character of litter size; development of ovary; RNA-Seq; differentially expressed genes
中文摘要:彭波半细毛羊是西藏自治区第一个国家级家畜新品种,对其产羔性状的研究有助于推动藏绵羊繁殖力研究工作。为研究产双羔彭波半细毛羊发情期内卵巢基因的特异调控模式,采用转录组测序对其差异表达基因进行了筛选和分析。结果发现,双羔组相对于单羔组有1 021个基因表达上调,1 468个基因表达下调。GO和KEGG分析发现,上调基因主要与细胞膜结构相关,下调基因主要与物质代谢等相关。其中,上调基因显著富集于与细胞膜相关的细胞粘附、细胞运动、物质运输、信号转导等生命活动。研究结果表明,双羔组卵巢内细胞的物质运输和信号转导频率加快,以促进卵泡的分化和发育;同时,双羔组卵巢内细胞的粘附及运动功能增强,有利于卵母细胞的发育和成熟卵子的排出。此外,卵巢中细胞粘附因子、TGF-beta信号通路和溶质运载蛋白家族基因的上调表达可作为彭波半细毛羊产双羔性状的潜在信号进行更深入的研究。研究结果不仅有利于对彭波半细毛羊产双羔性状的研究和利用,更有利于人们对藏绵羊繁育力的深入研究。
英文摘要:Pengbo semi fine wool sheep is the first national new breed of sheep in Tibet Autonomous Region, and the study on the litter size can facilitate the development of fecundity research in Tibetan sheep. In this study, RNA-sequencing was applied to investigate the regulation pattern of ovarian gene during the estrus period of Pengbo semi fine wool sheep producing twin lambs(dual group). The results showed that a total of 1 021 up-regulated and 1 468 down-regulated genes were detected in the dual group compared with the single group. GO and KEGG analysis revealed that the up-regulated genes were mainly related to cell membrane structure (cell adhesion, cell movement, material transport and signal transduction processes), while the down-regulated genes were mainly related to material metabolism. These results indicated that the frequency of material transport and signal transduction in the ovary of dual group contributed to the promotion of differentiation and the development of follicles. Moreover, the enhanced adhesion and motor function of the cells might facilitate the development of oocytes and the discharge of mature ovum. Further study concerning on the up-regulated cell adhesion molecules, TGF-beta signaling pathway and solute carrier family genes in ovary can contribute to developing potential marks for twin-lambs production. Therefore, this study is not only helpful to the litter size research of Pengbo semi fine wool sheep, but also contributes to the development of fecundity research in Tibetan sheep.
2020,39(2): 156-166 收稿日期:2019-10-08
分类号:S814.1
基金项目:西藏自治区重点研发及转化项目(XZ201801NB16);西藏自治区自然科学基金项目(XZ2018ZRG-37);国家绒毛用羊产业技术体系项目(CARS-39-33)
作者简介:张立(1986-),硕士,助理研究员,研究方向为绵羊遗传育种,E-mail:sagezhl@outlook.com
参考文献:
付绍印, 何小龙, 王标, 等. 2017. 不同产羔数绵羊性腺轴比较转录组研究[J]. 中国畜牧兽医, 44(2): 488-496.
贾建磊. 2015. 不同产羔性状绵羊卵巢组织差异表达蛋白质的筛选和分析[D]. 兰州: 甘肃农业大学.
兰道亮, 熊显荣, 位艳丽, 等. 2014. 基于RNA-Seq高通量测序技术的牦牛卵巢转录组研究: 进一步完善牦牛基因结构及挖掘与繁殖相关新基因[J]. 中国科学: 生命科学, 44(3): 307-317.
吕晓曼, 苏蕊, 张文广, 等. 2012. 绵羊繁殖性状相关基因的研究进展[J]. 中国畜牧兽医, 39(10): 185-190.
穆方方, 王鑫, 徐雯洁, 等. 2019. 彭波半细毛羊BMP15和BMPR-IB基因突变与其产羔性的相关分析[J]. 淮北师范大学学报(自然科学版), 40(3): 49-54.
肖国宏. 2014. miRNA-133b调控卵泡发育的分子机制[D]. 湖南: 南华大学.
央金. 2012. 彭波半细毛羊新品种种质特性研究[D]. 北京: 中国农业科学院.
杨广礼, 罗玉柱. 2004. 绵羊多胎性的研究与利用[J]. 甘肃农业大学学报, 39(1): 77-81.
杨永刚, 张梅, 胡耀中, 等. 2019. 基于转录组学的梅花鹿茸皮组织修复机制研究[J]. 四川动物, 38(4): 394-401.
应诗家. 2012. 黄体期不同饲喂量对湖羊卵泡发育、血液理化指标和卵泡内微环境的影响[D]. 南京: 南京农业大学.
张立岭. 2004. 绵羊的系统分类和种系发生[J]. 中国草食动物, 24(3): 43-44.
Chong Y, Liu G, Jiang X. 2019. Effect of BMPRIB gene on litter size of sheep in China: a meta-analysis[J/OL]. Animal Reproduction Science, 210: 106175[2019-08-10]. https://doi.org/10.1016/j.anireprosci.2019.106175.
Gasior K, Wagner NJ, Cores J, et al. 2019. The role of cellular contact and TGF-beta signaling in the activation of the epithelial mesenchymal transition (EMT)[J]. Cell Adhesion & Migration, 13(1): 63-75.
Kidder GM, Mhawi AA. 2002. Gap junctions and ovarian folliculogenesis[J]. Reproduction, 123(5): 613-620.
Love MI, Huber W, Anders S. 2014. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2[J]. Genome Biology, 15(12): 1-21.
Matikainen T, Perez GI, Zheng TS, et al. 2001. Caspase-3 gene knockout defines cell lineage specificity for programmed cell death signaling in the ovary[J]. Endocrinology, 142(6): 2468-2480.
Matzuk MM, Burns KH, Viveiros MM, et al. 2002. Intercellular communication in the mammalian ovary: oocytes carry the conversation[J]. Science, 296(5576): 2178-2180.
Miao XY, Luo QM, Qin XY. 2014. Genome-wide transcriptome analysis of mRNA and microRNAs in dorset and small tail han sheep to explore the regulation of fecundity[J]. Molecular and Cellular Endocrinology, 402(2015): 32-42.
Nilsson EE, Skinner MK. 2004. Kit ligand and basic fibroblast growth factor interactions in the induction of ovarian primordial to primary follicle transition[J]. Molecular and Cellular Endocrinology, 214(1-2): 19-25.
Sandoval A, Duran P, Gandini MA, et al. 2017. Regulation of L-type CaV1.3 channel activity and insulin secretion by the cGMP-PKG signaling pathway[J]. Cell Calcium, 66: 1-9.
Tang J, Hu W, Chen S, et al. 2019. The genetic mechanism of high prolificacy in small tail han sheep by comparative proteomics of ovaries in the follicular and luteal stages[J/OL]. Journal of Proteomics, 204: 103394[2019-08-02]. https://doi.org/10.1016/j.jprot.2019.103394.
Wigglesworth K, Lee KB, Emori C, et al. 2015. Transcriptomic diversification of developing cumulus and mural granulosa cells in mouse ovarian follicles[J]. Biology of Reproduction, 92(1): 23-37.
Yan C, Wang P, DeMayo J. 2001. Synergistic roles of bone morphogenetic protein 15 and growth differentiation factor 9 in ovarian function[J]. Molecular Endocrinology, 15(6): 854-866.
Zheng J, Wang Z, Yang H, et al. 2019. Pituitary transcriptomic study reveals the differential regulation of lncRNAs and mRNAs related to prolificacy in different FecB genotyping sheep[J/OL]. Genes, 10(2): 157[2019-08-10]. https://doi.org/10.3390/genes10020157.
Zheng X, Price CA, Tremblay Y, et al. 2008. Role of transforming growth factor-betal 1 in gene expression and activity of estradiol and progesterone-generating enzymes in FSH-stimulated bovine granulosa cells[J]. Reproduction, 136(4): 447-457.
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