英文摘要：The genes encoding the major histocompatibility complex (MHC) are important in the immune system of vertebrates. MHC-DOA gene is a non-classical gene of MHC class Ⅱ, of which exon 2 has rich polymorphisms. Hog deer (
Axis porcinus) is an extremely endangered species in China. In this study, total RNA was extracted from the blood of
A. porcinus and then the MHC-DOA gene was cloned by PCR from cDNA template. Meanwhile, the exon 2 of MHC-DOA genes in 38
A. porcinus from Chengdu Zoo were obtained by PCR and DNA-sequencing, followed by genetic polymorphism analysis. The results showed that the MHC-DOA gene of
A. porcinus contained a 753 bp open reading frame, encoding a protein with a length of 250 amino acid residues. Sequence analysis predicted that DOA protein had high amino acids similarity (98.4%) with
Cervus elaphus hippelaphus. A total of 10 haplotypes of DOA exon 2 were detected from the 38 individuals, indicating a medium level of genetic diversity in
A. porcinus. Nucleotides insert and deletion were observed in MHC-DOA exon 2 gene, and these resulted in the change of MHC-DOA amino acid sequence. This study has significant importance in understanding the population genetic structure and the protection of
A. porcinus in the future.
2020,39(1): 81-87 收稿日期：2019-03-14
分类号：Q959.842;Q987.1
基金项目：成都大熊猫繁育研究基金项目（CPF-2010-02）
作者简介：邵欢欢(1984-),男,博士,讲师,从事分子遗传学研究,E-mail:wsshforget@163.com;邓家波(1974-),男,硕士,高级兽医师,从事野生动物的研究,E-mail:565211989@qq.com
*通信作者：谭雪梅,讲师,主要从事动物分子遗传学研究,E-mail:txmyyf@scu.edu.cn
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