刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610065
电话:028-85410485; 15881112385
传真:028-85410485
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2019年第38卷第5期

MC1R基因多态性与豚鼠隐性黄毛色的相关性分析
Association Analysis of Polymorphism of MC1R Gene with Recessive Yellow-Coated Color in Cavia porcellus
赖伟宁1, 康建红1, 于丰1, 胡明月1, 闫守庆1, 祝万菊2*
点击:371次 下载:0次
DOI:10.11984/j.issn.1000-7083.20190114
作者单位:1. 吉林大学动物科学学院, 长春 130062;
2. 吉林大学动物医学学院, 长春 130062
中文关键字:豚鼠;MC1R基因;毛色
英文关键字:Cavia porcellus; MC1R gene; coat color
中文摘要:豚鼠Cavia porcellus的隐性黄毛色表型是由编码黑素皮质激素受体1(MC1R)的extension基因座位的等位基因e控制。本研究对野生型和黄毛色豚鼠MC1R基因位点所在区域进行PCR扩增与测序发现,在黄毛色豚鼠中存在1个2 760 bp的基因组缺失,该缺失涵盖了MC1R基因的整个编码区。采用三引物扩增体系对豚鼠MC1R基因缺失突变进行群体基因分型,在随机选择的58只野生型个体中,36只为EE纯合子,22只为Ee杂合子,而31只黄毛色个体均为ee纯合子;在15只测交后代中,8只黄毛色个体均为ee纯合子,而7只野生型个体均为Ee杂合子。基因分型结果表明,MC1R基因2 760 bp的缺失与隐性黄毛色完全相关。本研究为进一步探究MC1R基因在哺乳动物毛色遗传机制中的作用以及豚鼠的分子标记辅助育种提供了理论依据。
英文摘要:The recessive yellow-coated color in guinea pigs (Cavia porcellus) is determined by the e allele at extention locus encoding melanocortin receptor 1 (MC1R). Comparing to the wild-type guinea pigs, a 2 760 bp deletion including the entire coding region of MC1R gene was identified in the yellow-coated individuals by PCR amplification and sequencing. The results of PCR-based genotyping using 3 primers showed that among the 58 randomly selected wild-type guinea pigs, 36 were EE genotype and 22 were Ee, and all the 31 yellow-coated individuals were ee homozygous. Among the 15 test-crossing offsprings, all the 8 yellow-coated individuals were ee homozygous, while the remaining 7 wild-type individuals were Ee heterozygous, indicating the absolute correlation of the 2 760 bp deletion region of MC1R gene with the recessive yellow-coated color in C. porcellus. These results provide theoretical basis for further exploration of the function of MC1R gene in the genetic mechanism of mammalian coat color and molecular marker-assisted breeding in C. porcellus.
2019,38(5): 506-511 收稿日期:2019-04-01
分类号:S813.1
基金项目:国家自然科学基金项目(31601004);国家大学生创新创业训练计划项目(2018A8506)
作者简介:赖伟宁(1996-),女,本科生,E-mail:2390221455@qq.com
*通信作者:祝万菊,高级工程师,研究方向:动物机能学实验技术,E-mail:wjzhu@jlu.edu.cn
参考文献:
刘迪文, 谢敏, 陈雁虹, 等. 2017. Zmu-1:DHP近交系豚鼠的培育及其分子遗传结构初步鉴定[J]. 中国实验动物学报, 25(1):90-96.
杨永升, 李宁, 邓学梅, 等. 2004. 黑素皮质素受体1——哺乳动物黑色素形成中的关键基因[J]. 遗传, 26(4):544-550.
赵珊珊, 肖宁, 李志鹏, 等. 2018. 马MC1R基因多态位点与毛色相关性分析[J]. 畜牧兽医学报, 49(8):1605-1616.
Bauer A, Kehl A, Jagannathan V, et al. 2018. A novel MLPH variant in dogs with coat colour dilution[J]. Animal Genetics, 49(1):94-97.
Druml T, Grilz-Seger G, Neuditschko M, et al. 2018. Novel insights into Sabino1 and splashed white coat color patterns in horses[J]. Animal Genetics, 49(3):249-253.
Everts RE, Rothuizen J, van Oost BA. 2000. Identification of a premature stop codon in the melanocyte-stimulating hormone receptor gene (MC1R) in labrador and golden retrievers with yellow coat colour[J]. Animal Genetics, 31(3):194-199.
Fontanesi L, Dall'olio S, Beretti F, et al. 2011. Coat colours in the Massese sheep breed are associated with mutations in the agouti signalling protein (ASIP) and melanocortin 1 receptor (MC1R) genes[J]. Animal, 5(1):8-17.
Fontanesi L, Tazzoli M, Beretti F, et al. 2006. Mutations in the melanocortin 1 receptor (MC1R) gene are associated with coat colours in the domestic rabbit (Oryctolagus cuniculus)[J]. Animal Genetics, 37(5):489-493.
García-Borrón JC, Sánchez-Laorden BL, Jiménez-Cervantes C. 2005. Melanocortin-1 receptor structure and functional regulation[J]. Pigment Cell Research, 18(6):393-410.
Ibsen HL. 1919. Tricolor inheritance. IV. The triple allelo-morphic series in guinea-pigs[J]. Genetics, 4(6):597-606.
Kijas JM, Wales R, Törnsten A, et al. 1998. Melanocortin receptor 1 (MC1R) mutations and coat color in pigs[J]. Genetics, 150(3):1177-1185.
Klungland H, Våge DI, Gomez-Raya L, et al. 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination[J]. Mammal Genome, 6(9):636-639.
Langevin M, Synkova H, Jancuskova T, et al. 2018. Merle phenotypes in dogs-SILV SINE insertions from Mc to Mh[J]. PLoS ONE, 13(9):e0198536. DOI:10.1371/journal.pone.0198536.
Marklund L, Moller MJ, Sandberg K, et al. 1996. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses[J]. Mammal Genome, 7(12):895-899.
Newton JM, Wilkie AL, He L, et al. 2000. Melanocortin 1 receptor variation in the domestic dog[J]. Mammal Genome, 11(1):24-30.
Rees JL. 2000. The melanocortin 1 receptor (MC1R):more than just red hair[J]. Pigment Cell Research, 13(3):135-140.
Robbins LS, Nadeau JH, Johnson KR, et al. 1993. Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function[J]. Cell, 72(6):827-834.
Thomas AJ, Erickson CA. 2008. The making of a melanocyte:the specification of melanoblasts from the neural crest[J]. Pigment Cell & Melanoma Research, 21(6):598-610.
Vidal O. 2018. Deleterious mutations of MC1R in guinea pig[J]. Animal Genetics, 49(5):498-499.
Wright S. 1927. The effects in combination of the major color-factors of the guinea pig[J]. Genetics, 12(6):530-569.
Yu F, Jiao S, Lai W, et al. 2018a. Conserved aspartate-to-glycine mutation in tyrosinase is associated with albino phenotype in domestic guinea pigs (Cavia porcellus)[J]. Animal Genetics, 49(4):354-355.
Yu F, Liu Z, Jiao S, et al. 2018b. A nonsense mutation in the FGF5 gene is associated with the long-coated phenotype in domestic guinea pigs (Cavia porcellus)[J]. Animal Genetics, 49(3):269. DOI:10.1111/age.12656.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2019四川动物》编辑部 蜀ICP备08107403号-3
您是本站第7871199名访问者

川公网安备 51010702000173号