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基于转录组学的梅花鹿茸皮组织修复机制研究
Study on Tissue Repair Mechanism of Sika Deer Antler Velvet Skins Based on Transcriptomics
杨永刚1, 张梅2, 胡耀中3, 赵雨3, 赵大庆3, 幺宝金3*
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DOI:10.11984/j.issn.1000-7083.20190005
作者单位:1. 长春中医药大学附属医院, 长春 130117;
2. 长春中医药大学创新实践中心, 长春 130117;
3. 长春中医药大学吉林省人参科学研究院, 长春 130117
中文关键字:梅花鹿;生长中心;茸皮;转录组学;组织损伤修复
英文关键字:Cervus nippon; growth center; velvet skin; transcriptomics; tissue repair
中文摘要:为研究不同生长时期梅花鹿东北亚种Cervus nippon hortulorum鹿茸茸皮差异基因表达变化,以期为鹿茸生长机制及组织修复的临床治疗提供理论依据,本研究采用Illumina测序技术和生物信息学方法对快速生长期和骨化期鹿茸生长中心茸皮进行转录组测序和差异基因表达分析。从快速生长期和骨化期茸皮中分别获得45 422 254条和44 530 430条clean reads,测序质量值分别为98.50和98.39;通过Trinity组装,分别获得44 352条和43 194条组装序列,平均长度分别为977 nt和1 135 nt。通过数据库比对和差异基因表达分析筛选出生长因子7种、转录因子25种和胶原分子6种。在快速生长期鉴定出多种参与组织修复的重要生长因子和转录因子,包括Pdgfa、Tgfβ3、Egfl7、Vegfb、Sox4、Sox12、Scx、E4f1、Tcf4和Elf4等。
英文摘要:We aimed to study the gene expression pattern of the velvet skin of sika deer (Cervus nippon hortulorum) antler at different growth stages, and to provide theoretical basis for the growth mechanism of the antler as well as the clinical treatments of tissue repair. Illumina RNA sequencing and bioinformatic tools were conducted to analyze the gene expression pattern of velvet skins at rapid growth and ossification stages. Finally, 45 422 254 and 44 530 430 clean reads with Q20 values of 98.50 and 98.39 were generated from the rapid growth and the ossification stages, respectively. A total of 44 352 and 43 194 Unigenes with average lengths of 977 nt and 1 135 nt were obtained by using Trinity software. We identified 7 growth factors, 25 transcription factors and 6 collagens by BLAST against the protein database. Among these genes, a series of genes (including Pdgfa, Tgfβ3, Egfl7, Vegfb, Sox4, Sox12, Scx, E4f1, Tcf4 and Elf4) which play pivotal roles in regulating tissue repair were identified. Our current study provides a data basis for the mechanistic studies on antler growth and tissue repair.
2019,38(4): 394-401 收稿日期:2019-01-05
分类号:Q95-33;Q786
基金项目:国家重点研发计划项目(2018YFC1706605);吉林省教育厅“十三五”科学技术研究项目(JJKH20181269KJ;JJKH20181272KJ;JJKH20170721KJ);吉林省科技厅优秀青年人才基金项目(20170520044JH)
作者简介:杨永刚(1976-),男,博士,助理研究员,研究方向为鹿茸生长机制及健康产品开发,E-mail:ccyyg1976@sina.com
*通信作者:幺宝金,E-mail:ybj19830928@sina.com
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