Grx-1基因敲除鼠的繁殖及子代基因型鉴定
Breeding and Identification of Glutaredoxin-1 Knockout Mice
刘秋彤，韩文莉，郭春宝，邓春*
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DOI：
作者单位：(重庆医科大学附属儿童医院，重庆400014）
中文关键字：谷氧还蛋白；基因敲除；基因型鉴定；氧化应激
英文关键字：glutaredoxin; gene knockout; gene identification; oxidative stress
中文摘要：目的 探讨glutaredoxin-1(Grx-1)基因敲除小鼠的优化繁殖及子代鼠的鉴定方法，为进一步研究Grx-1在支气管肺发育不良(BPD)中的作用奠定基础。方法 将从美国哈佛医学院引进的纯合子Grx-1基因敲除小鼠与野生型小鼠进行交配后得到的子一代小鼠同代间相互交配，繁殖出的子二代中将出现纯合子、杂合子以及野生型3种基因型。从出生起观察其生长发育情况，2周龄时剪尾提取基因组DNA，用PCR方法扩增目的基因片段，琼脂糖凝胶电泳结果判定基因型。结果 Grx-1纯合子小鼠的饲养繁殖取得成功，获得了一批Grx-1基因敲除纯合子小鼠。结论 正确的饲养繁殖以及鉴定方法是获得Grx-1基因敲除纯合子小鼠的有效途径，为相关研究提供动物实验模型奠定了基础。
英文摘要：Objective To explore the optimal way of breeding and identification of glutaredoxin-1（Grx-1）knockout mice and establish an experimental animal model for further study on the role of Grx-1 gene. Methods Breeding homozygote genotype of Grx-1 gene knockout mice (Harvard Medical School) were copulated with wild-type C57BL/6J mice, and then the heterozygous were used for mating with each other. To observe the growth and development of offspring, genomic DNA was extracted from the tail of two-week-old mice. PCR was employed to amplify the Grx-1 gene fragment, and the gene type was presented by electrophoresis. Results The results showed that the breeding and reproducing were successful and three genotype offspring including wild-type, heterozygous and homozygous knockout mice were finally obtained. Conclusion It is feasible to obtain the homozygous Grx-1 knockout mice by inbreeding of the heterozygotes. This approach allows breeding of sufficient Grx-1 knock-out mice for relevant research.