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多次注射乌拉坦诱导的BALB C及C57BL 6J小鼠肺癌模型的比较
Comparative Study of Lung Cancer Models on BALB C and C57BL 6J Mice Induced by Multiple Urethane Injections
陈婷1,2,王双3,张恋1,2,雷晓琴1,2,吴诗诗1,2,练雪梅1,2*
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DOI:
作者单位:(1. 重庆医科大学公共卫生与管理学院,营养与食品卫生学教研室,重庆400016;2. 重庆医科大学脂质研究中心,重庆 400016;3. 浙江省台州医院临床营养治疗中心,浙江台州317000)
中文关键字:乌拉坦;BALB/C;C57BL/6J;肺癌动物模型;比较研究
英文关键字:urethane; BALB/C; C57BL/6J; lung cancer animal model; comparative study
中文摘要:目的 探讨一种稳定的多次乌拉坦注射诱导小鼠肺癌模型的构建方法,比较BALB/CC57BL/6J小鼠对该肺癌模型的敏感性。方法 10BALB/C小鼠及10C57BL/6J小鼠适应性饲养3周, 随后每只小鼠分别被给予每周1次腹腔注射1 g/kg体重乌拉坦,连续注射10周,继续喂养15周后处死小鼠取肺组织。由3名不同的检测者在解剖显微镜下观察计数肺组织表面肿瘤数目并以直径记录肿瘤大小;HE染色检测肺组织病理变化。结果 多次乌拉坦注射诱导的BALB/CC57BL/6J小鼠肺癌发生率均为10/10(100%)BALB/C小鼠荷瘤数明显多于C57BL/6J小鼠(P<0.01),同时,直径也大于C57BL/6J小鼠(P<0.05)HE染色显示多次乌拉坦注射诱导的肺癌有非典型性腺瘤增生及腺瘤两种病变类型 结论 BALB/CC57BL/6J小鼠均可以作为多次注射乌拉坦诱导性肺癌模型的动物,BALB/C小鼠对该肺癌模型的敏感性高于C57BL/6J小鼠。
英文摘要:Objective To explore a method for establishing stable mouse model of lung cancer, and compare the susceptibility of BALB/C and C57BL/6J mice for multiple urethane injections-induced lung carcinogenesis. Methods Ten BALB/C and ten C57BL/6J mice were normally feed for 3 weeks, and then received 1 g/kg urethane by weekly intraperitoneal injection for 10 weeks, followed by 15 weeks’ latency period. Finally, all the mice were sacrificed and the lung tissues were isolated. During the autopsy procedure, the tumor lesions on lung surfaces were carefully counted by three blinded readers, and the lesion diameter was measured with the aid of digital calipers under a dissecting microscope; HE staining was used to examine the pathological characteristics of lung tissues. Results The results showed that the incidences of both BALB/C and C57BL/6J mice were 10/10(100%) ; The number of BALB/C mice-bearing lung tumors was significantly more than that of C57BL/6J mice (P<0.01), meanwhile the diameter of BALB/C mice-bearing tumor was larger than that of C57BL/6J mice (P<0.05); The results of HE staining showed that atypical adenomatous hyperplasia and adenomas were the two main pathological types of multiple urethane injections-induced lung cancer model in this study. Conclusion Both BALB/C and C57BL/6J mice could be used as model animals of multiple urethane injections-induced lung cancer, and the susceptibility of BALB/C mice was higher than that of C57BL/6J mice.
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